Awake-1
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB20146 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR1904924_1.fastq.gz | Fastqsanger.gz | |||
| ERR1904924_2.fastq.gz | Fastqsanger.gz | |||
| ERR1904925_1.fastq.gz | Fastqsanger.gz | |||
| ERR1904925_2.fastq.gz | Fastqsanger.gz |
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Phosphoproteomic analysis was carried out by using following process.
Isolated barley seed embryo was used for protein extraction. Trypsin digestion and phosphopeptide enrichment by HAMMOC method were performed, then purified peptide sample was analyzed by TripleTOF5600 (AB-SCIEX).
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Project description:The role of non-CG methylation in seed development and dormancy remains unknown. There are four genes in charge of non-CG methylation in Arabidopsis: drm1, drm2, cmt2 and cmt3. The majority of non-CG methylation in vegetative tissues, leaf, is gone in homozygous ddcc mutant line (Hume et al., 2014). To uncover the possible role of non-CG DNA methylation in seed development and dormancy, we characterized the methylome of ddcc mutant in Arabidopsis postmature-green-stage seed and dry seed using Illumina sequencing. Meanwhile, vegetative tissue, leaves from 3 week plant with ddcc mutant and from wild-type, and postmature-green-stage seed and dry seed from wild-type plant were used as control.
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