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Opposing Role of Dpf2 and Eed on Differentiation of Mouse Embryonic Stem Cells via Regulating Tbx3 (RNA-seq)

ABSTRACT: Opposing Role of Dpf2 and Eed on Differentiation of Mouse Embryonic Stem Cells via Regulating Tbx3 (RNA-seq)

PROVIDER: PRJEB23280 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2188113_1.fastq.gz	Fastqsanger.gz
	ERR2188113_2.fastq.gz	Fastqsanger.gz
	ERR2188114_1.fastq.gz	Fastqsanger.gz
	ERR2188114_2.fastq.gz	Fastqsanger.gz
	ERR2188115_1.fastq.gz	Fastqsanger.gz

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Opposing Role of Dpf2 and Eed on Differentiation of Mouse Embryonic Stem Cells via Regulating Tbx3 (4C)

Project description:4C experiments of wild-type and Dpf2 -/- cells to investigate the effect of Dpf2 on the chromatin contacts of Tbx3 locus.

2018-11-30 | E-MTAB-6197 | biostudies-arrayexpress

Opposing Role of Dpf2 and Eed on Differentiation of Mouse Embryonic Stem Cells via Regulating Tbx3 (4C)

Project description:Opposing Role of Dpf2 and Eed on Differentiation of Mouse Embryonic Stem Cells via Regulating Tbx3 (4C)

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ChIP-Seq to investigate the opposing role of Dpf2 and Eed on differentiation of Mouse Embryonic Stem Cells via regulating Tbx3

Project description:ChIP-Seq to investigate the opposing role of Dpf2 and Eed on differentiation of Mouse Embryonic Stem Cells via regulating Tbx3

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Opposing Role of Dpf2 and Eed on Differentiation of Mouse Embryonic Stem Cells via Regulating Tbx3 (ChIP-seq)

Project description:ChIP-seq experiments of 3xFlag-Dpf2, Oct4, Sox2 and several histone marks were performed in wild-type and Dpf2 -/- cells to investigate the effect of Dpf2 on the binding of those factors.

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Opposing Role of Dpf2 and Eed on Differentiation of Mouse Embryonic Stem Cells via Regulating Tbx3 (RNA-seq)

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Genome-wide occupancy profile of Tbx3 in adult mouse whole heart

Project description:The contraction pattern of the heart relies on the activation and conduction of the electrical impulse. Perturbations of cardiac conduction have been associated with congenital and acquired arrhythmias as well as cardiac arrest. The pattern of conduction depends on the regulation of heterogeneous gene expression by key transcription factors and transcriptional enhancers. Here, we assessed the genome-wide occupation of conduction system–regulating transcription factors TBX3 in the mouse heart, uncovering cardiac enhancers throughout the genome. Examination of the cardiac transcription factor Tbx3 in adult mouse heart

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EED is required for Mouse Primordial Germ Cell Differentiation in the Embryonic Gonad

Project description:Development of Primordial germ cells (PGCs) is required for reproduction. During PGC development in mammals, major epigenetic remodeling occurs which is hypothesized to establish an epigenetic landscape for sex-specific germ cell differentiation and gametogenesis. In order to address the role of Embryonic Ectoderm Development (EED) and Histone 3 lysine 27 trimethylation (H3K27me3) in this process, we created a conditional deletion in EED and show that EED is essential for regulating the timing of sex-specific PGC differentiation in both ovaries and testes, as well as X chromosome dosage decompensation in testes. Integrating chromatin and whole genome bisulfite sequencing of epiblast and PGCs, we identified a poised repressive signature of H3K27me3/DNA methylation which we propose is established in the epiblast where EED and DNMT1 interact. Thus, EED joins DNMT1 in regulating the timing of sex-specific PGC differentiation during the critical window when the gonadal niche cells specialize into an ovary or testis.

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A dynamic role of Tbx3 in the pluripotency circuitry

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