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RNA-seq expression profiling of OCI-AML2 and OCI-AML3 cells upon CRIPSR/Cas9 –mediated disruption of DDX3X RNA binding domain

ABSTRACT: RNA-seq expression profiling of OCI-AML2 and OCI-AML3 cells upon CRIPSR/Cas9 –mediated disruption of DDX3X RNA binding domain

PROVIDER: PRJEB23614 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2204250_1.fastq.gz	Fastqsanger.gz
	ERR2204250_2.fastq.gz	Fastqsanger.gz
	ERR2204241_1.fastq.gz	Fastqsanger.gz
	ERR2204241_2.fastq.gz	Fastqsanger.gz
	ERR2204251_1.fastq.gz	Fastqsanger.gz

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RNA-seq expression profiling of OCI-AML2 and OCI-AML3 cells upon CRISPR/Cas9 –mediated disruption of DDX3X RNA binding domain

Project description:Human acute myeloid leukemia cell lines OCI-AML2 and OCI-AML3 were used in a CRISPR/Cas9-mediated approach to specifically target DDX3X’s gene sequences encoding the RNA binding domain of the helicase. DDX3X RNA binding domain is bipartite in the two halves of the helicase core. sgRNAs were designed to target both halves of the domain (named RNA binding domain A and B – RBDA and RBDB). We performed RNA-seq to observe the gene expression changes in both OCI-AML2 and OCI-AML3 cell lines following the not-combined CRISPR/Cas9 –mediated targeting of both regions of the DDX3X RNA binding domain. Control CRISPR/Cas9 performed with no sgRNA expressing vector (named “empty vector”) was performed in both cell lines. The latter condition was used as a control for gene expression changes analysis, for each cell line.

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