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Antarctic Snow Algae 2014/15

ABSTRACT: Antarctic Snow Algae 2014/15 16S and 18S Illumina Seq of snow algae communities around Ryder Bay, Antarctica

PROVIDER: PRJEB23732 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	ERR2226420_1.fastq.gz	Fastqsanger.gz
	ERR2226420_2.fastq.gz	Fastqsanger.gz
	ERR2226470_1.fastq.gz	Fastqsanger.gz
	ERR2226470_2.fastq.gz	Fastqsanger.gz
	ERR2226421_1.fastq.gz	Fastqsanger.gz

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Antarctic Snow Algae 2014/15

Project description:Antarctic Snow Algae 2014/15 16S and 18S Illumina Seq of snow algae communities around Ryder Bay, Antarctica

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RNA-seq of iron-rich pelagic aggregates (iron snow) in 2017 and 2018

Project description:Iron-rich pelagic aggregates (iron snow) were collected directly onto silicate glass filters using an electronic water pump installed below the redoxcline. RNA was extracted and library preparation was done using the NEBNext Ultra II directional RNA library prep kit for Illumina. Data was demultiplied by GATC sequencing company and adaptor was trimmed by Trimgalore. After trimming, data was processed quality control by sickle and mRNA/rRNA sequences were sorted by SortmeRNA. mRNA sequences were blast against NCBI-non redundant protein database and the outputs were meganized in MEGAN to do functional analysis. rRNA sequences were further sorted against bacterial/archeal 16S rRNA, eukaryotic 18S rRNA and 10,000 rRNA sequences of bacterial 16S rRNA, eukaryotic 18S rRNA were subset to do taxonomy analysis.

2021-10-15 | E-MTAB-9686 | biostudies-arrayexpress

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