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Hypothalamic DNA-methylation differences in Red Junglefowl divergently selected for reduced fear of humans

ABSTRACT: Hypothalamic DNA-methylation differences in Red Junglefowl divergently selected for reduced fear of humans

PROVIDER: PRJEB24705 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2275170.fastq.gz	Fastqsanger.gz
	ERR2275171.fastq.gz	Fastqsanger.gz
	ERR2275172.fastq.gz	Fastqsanger.gz
	ERR2275173.fastq.gz	Fastqsanger.gz
	ERR2275174.fastq.gz	Fastqsanger.gz

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Similar Datasets

Hypothalamic DNA-methylation differences in Red Junglefowl divergently selected for reduced fear of humans

Project description:Domestication of animals causes large phenotypic alterations during a brief evolutionary window of time. These alterations are caused by genomic variation, yet the prevalence of modified traits is larger than expected if caused only by classical genetics and mutations. Two selection lines of Red Junglefowl (ancestors of modern chickens), bred for either high or low fear of human for five generations, were used to study the differences in hypothalamic DNA methylation between the two populations.

2018-05-29 | E-MTAB-6407 | biostudies-arrayexpress

Epigenetics and early domestication: differences in hypothalamic DNA methylation between red junglefowl divergently selected for high or low fear of humans.

Project description:BACKGROUND:Domestication of animals leads to large phenotypic alterations within a short evolutionary time-period. Such alterations are caused by genomic variations, yet the prevalence of modified traits is higher than expected if they were caused only by classical genetics and mutations. Epigenetic mechanisms may also be important in driving domesticated phenotypes such as behavior traits. Gene expression can be modulated epigenetically by mechanisms such as DNA methylation, resulting in modifications that are not only variable and susceptible to environmental stimuli, but also sometimes transgenerationally stable. To study such mechanisms in early domestication, we used as model two selected lines of red junglefowl (ancestors of modern chickens) that were bred for either high or low fear of humans over five generations, and investigated differences in hypothalamic DNA methylation between the two populations. RESULTS:Twenty-two 1-kb windows were differentially methylated between the two selected lines at p?<?0.05 after false discovery rate correction. The annotated functions of the genes within these windows indicated epigenetic regulation of metabolic and signaling pathways, which agrees with the changes in gene expression that were previously reported for the same tissue and animals. CONCLUSIONS:Our results show that selection for an important domestication-related behavioral trait such as tameness can cause divergent epigenetic patterns within only five generations, and that these changes could have an important role in chicken domestication.

| S-EPMC5880090 | biostudies-literature

Is domestication driven by reduced fear of humans? Boldness, metabolism and serotonin levels in divergently selected red junglefowl (Gallus gallus).

Project description:Domesticated animals tend to develop a coherent set of phenotypic traits. Tameness could be a central underlying factor driving this, and we therefore selected red junglefowl, ancestors of all domestic chickens, for high or low fear of humans during six generations. We measured basal metabolic rate (BMR), feed efficiency, boldness in a novel object (NO) test, corticosterone reactivity and basal serotonin levels (related to fearfulness) in birds from the fifth and sixth generation of the high- and low-fear lines, respectively (44-48 individuals). Corticosterone response to physical restraint did not differ between selection lines. However, BMR was higher in low-fear birds, as was feed efficiency. Low-fear males had higher plasma levels of serotonin and both low-fear males and females were bolder in an NO test. The results show that many aspects of the domesticated phenotype may have developed as correlated responses to reduced fear of humans, an essential trait for successful domestication.

| S-EPMC4614427 | biostudies-literature

Oprl1 regulation of amygdala-dependent fear in mice and humans with PTSD

Project description:Analysis of amygdala RNA expression 2 hours after auditory fear conditioning in mice with and without previous exposure to acute immobilization stress Total RNA from three groups was obtained: 1) Home Cage (HC) 2) Fear Conditioning (FC) 3) Immobilization (IMO) + FC

2013-04-01 | E-GEOD-44944 | biostudies-arrayexpress

Selection for and against fearfulness in chicken (Gallus gallus) alter transcriptome profiles in only a few generations

Project description:Domesticated animals all show the same patterns regarding phenotypic traits and behaviour, collectively known as the domestic phenotype. All domestic chicken come from the red junglefowl. By keeping three separate populations of junglefowl and selecting for high, low or intermediate fear responses towards humans, the goal is to in the low fear group start to unlock domestic phenotypes.

2016-12-05 | E-MTAB-4113 | biostudies-arrayexpress

Targeting acetyl-CoA metabolism attenuates the formation of fear memories through reduced activity-dependent histone acetylation

Project description:Histone acetylation is a key component in the consolidation of long-term fear memories, which are models for highly resilient and durable memory. Histone acetylation is fueled by acetyl-CoA and recently, nuclear-localized metabolic enzymes that produce this metabolite have emerged as direct and local regulators of histone acetylation. In particular, Acetyl-coA synthetase 2 (ACSS2) mediates histone acetylation in the mouse hippocampus. However, whether ACSS2 regulates long-term fear memory remains to be determined. Here, we show that Acss2 knockout is well-tolerated in mice, yet the Acss2 null mouse exhibits reduced acquisition of long-term fear memory. Loss of Acss2 leads to reductions in both histone acetylation and expression of critical learning and memory-related genes in the dorsal hippocampus, specifically following fear conditioning. Further, systemic administration of blood-brain-barrier (BBB)-permeable Acss2 inhibitors during the consolidation window reduces fear memory formation in mice and rats, and reduces anxiety in a predator-scent-stress (PSS) paradigm. Our findings suggest that nuclear acetyl-CoA metabolism via ACSS2 plays a critical, previously unappreciated role in the formation of fear memories.

2022-07-19 | GSE199887 | GEO

Reduced a-MSH underlies hypothalamic endoplasmic reticulum stress-induced hepatic gluconeogenesis

Project description:Alterations in endoplasmic reticulum (ER) homeostasis have been implicated in the pathophysiology of obesity and type-2 diabetes (T2D). Acute ER stress induction in the hypothalamus produces glucose metabolism perturbations. However, the neurobiological basis linking hypothalamic ER stress with abnormal glucose metabolism remains unknown. Here we report that genetic and induced models of hypothalamic ER stress are associated with alterations in systemic glucose homeostasis due to increased gluconeogenesis (GNG) independent of body weight changes. Defective alpha melanocyte-stimulating hormone (a-MSH) production underlies this metabolic phenotype, as pharmacological strategies aimed at rescuing hypothalamic a-MSH content reversed this phenotype at metabolic and molecular level. Collectively, our results posit defective a-MSH processing as a fundamental mediator of enhanced GNG in the context of hypothalamic ER stress, and establish a-MSH deficiency in proopiomelanocortin (POMC) neurons as a potential contributor to the pathophysiology of T2D. Total RNA was extracted from whole-liver of 6-week old control (3 biological replicates) and POMCMfn2KO mice (5 biological replicates)

2015-06-18 | E-GEOD-62263 | biostudies-arrayexpress

Amygdala-Dependent Fear Is Regulated by Oprl1 in Mice and Humans with PTSD

Project description:Analysis of amygdala RNA expression 2 hours after auditory fear expression test in mice with and without previous exposure to acute immobilization stress.

2013-04-01 | GSE44944 | GEO

Selection for and against fearfulness in chicken (Gallus gallus) alter transcriptome profiles in the different selection lines in the cerebral hemisphere

2017-02-28 | E-MTAB-4741 | biostudies-arrayexpress

Hepatic transcriptional profiling of juvenile broiler chickens divergently selected for abdominal fatness or leanness

Project description:Chickens divergently selected for either high abdominal fat content (fat genotype) or low abdominal fat content (lean genotype) at SRA-INRA, France were used to profile hepatic gene expression during juvenile development (1 to 11 weeks of age) and to identify differentially expressed genes associated with genotype and age. The fat line (FL) and lean line (LL) chickens are different in various phenotypic and metabolic measurements, including abdominal fatness, plasma glycemia and T3. The FL and LL chickens represent unique models for characterizing biomedical and agricultural traits. The Del-Mar 14K Chicken Integrated Systems microarrays were used for a transcriptional scan in liver during juvenile development using a balanced block hybridization design. Log2-transformed fluorescence intensities were analyzed with a two-stage mixed model. A total of 905 differentially expressed "functional" genes were identified (FDR<0.10). The greatest number of differentially expressed genes (400) was detected at 7 weeks of age. The differentially expressed genes include metabolic enzymes, acute phase proteins, growth factors, immune factors and transcription factors involved in various pathways. Several of the functional genes are also identified as positional candidate genes within QTLs in an F2 population established from an intercross between the FL and LL lines. Keywords: Divergently selected chickens, fatness, transcriptional profiling, differentially expressed genes

2007-09-01 | GSE8812 | GEO

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