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Genome resequencing of pigmented rice varieties

ABSTRACT: Leaves from four pigmented rice varieties were sequenced using Illumina Hi-seq 4000 sequencing technology. Approximately 30x depth of sequencing for each varieties.

PROVIDER: PRJEB29070 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR2831550_1.fastq.gz	Fastqsanger.gz
	ERR2831550_2.fastq.gz	Fastqsanger.gz
	ERR2831551_1.fastq.gz	Fastqsanger.gz
	ERR2831551_2.fastq.gz	Fastqsanger.gz
	ERR2831548_1.fastq.gz	Fastqsanger.gz

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SWATH-based quantitative proteomics data derived from whole grain of Malaysian local pigmented and non-pigmented rice varieties

Project description:Information about protein expression in rice grain across both pigmented and non-pigmented rice varieties is still relatively scarce. The data provided here represent proteomic data obtained from selected 6 Malaysian local rice varieties with varying pigmentations (black, red and white). The selected pigmented rice varieties such as black (BALI and Pulut hitam 9) and red rice (MRQ100 and MRM16) have shown high antioxidant activities and non-pigmented rice (MRQ76 and MR297) contain amino acid and micronutrient contents. This project aimed to obtain global protein expression profile as well as differential protein expression between the selected pigmented and non-pigmented rice varieties particularly proteins with their functions responsible for nutritional (i.e. antioxidant, folate and low glycaemic index) and quality (i.e. aromatic) traits. Integration of this proteomics dataset with other available in-house omics data could facilitate the identification of significant functional markers related to nutritional and quality traits. Total proteins were prepared from dehusked matured seeds harvested from three different rice plants of each variety (3 protein samples per variety). The proteins were trypsin digested before subjected to SWATH-MS proteomics analysis. Proteins were identified by matching tandem mass (MS/MS) spectra from both 1D and 2D IDA to Oryza sativa japonica and indica rice databases available at UniProt by using ProteinPilot software (v4.2) (AB Sciex). Quantification of proteins was carried out by determining protein peak areas extracted from SWATH analysis data sets using PeakView (v2.1) (AB Sciex) software. Differentially expressed protein between varieties were identified using T-test analysis with a set threshold for fold change ± 1.5 and p‐value < 0.05.

2020-04-27 | PXD018338 | Pride

Data on genome resequencing of pigmented and non-pigmented Malaysian rice varieties.

Project description:The genomics and genetic data of pigmented and non-pigmented Malaysian rice varieties are still limited. Hence, we performed the genome resequencing of two black rice varieties (Bali, Pulut Hitam 9), two red rice varieties (MRM16, MRQ100) and two white rice varieties (MR297 and MRQ76) using Illumina HiSeq 4000 platform with 30x sequencing coverage. We aimed to identify and annotate single nucleotide polymorphisms (SNPs) from the genome of these four pigmented and two non-pigmented rice varieties. The potential SNPs will be used in developing the functional SNP markers related to nutritional (i.e. antioxidant, folate, amylose) and quality (i.e. aromatic) traits. Raw data of the pigmented and non-pigmented rice varieties have been deposited into the European Nucleotide Archive (ENA) database with accession number PRJEB29070 and PRJEB32344, respectively.

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Transcriptome analysis of pigmented and non-pigmented rice grain - focusing on antioxidant and micronutrient perspectives.

Project description:Transcriptome sequencing of pigmented and non-pigmented rice grain using Illumina HiSeq 4000 platform.

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Multi-omics resources for targeted agronomic improvement of pigmented rice

Project description:<p>Pigmented rice (<em>Oryza sativa L.</em>) is a rich source of nutrients, but pigmented lines typically have long life cycles and limited productivity. Here we generated genome assemblies of 5 pigmented rice varieties and evaluated the genetic variation among 51 pigmented rice varieties by resequencing an additional 46 varieties. Phylogenetic analyses divided the pigmented varieties into four varietal groups: Geng-japonica, Xian-indica, circum-Aus and circum-Basmati. Metabolomics and ionomics profiling revealed that black rice varieties are rich in aromatic secondary metabolites. We established a regeneration and transformation system and used CRISPR-Cas9 to knock out three flowering time repressors (Hd2, Hd4 and Hd5) in the black Indonesian rice Cempo Ireng, resulting in an early maturing variety with shorter stature. Our study thus provides a multi-omics resource for understanding and improving Asian pigmented rice.</p>

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Whole genome resequencing of MR297 and MRQ76 rice varieties.

Project description:Whole genome resequencing of MR297 and MRQ76 rice varieties using Illumina HiSeq 4000 sequencing technology.

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Transcription profiling of pigmented and non-pigmented tubers of Solanum tuberosum

Project description:Transcriptional analysis of pigmented and non-pigmented potato tuber flesh using POCI 44k microarrays

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Transcription profiling of pigmented and non-pigmented tubers of Solanum tuberosum

Project description:Transcriptional analysis of pigmented and non-pigmented potato tuber flesh using POCI 44k microarrays

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Gene expression of the pigmented and non-pigmented epithelia of the ciliary body of human healthy eyes

Project description:The ciliary body (CB) of the human eye consists of the non-pigmented (NPE) and pigmented (PE) neuro-epithelia. We investigated the gene expression of the NPE and PE, to shed light on the molecular mechanisms underlying the most important functions of the CB. Therefore we isolated NPE and PE cells from seven healthy human donor eyes using laser dissection microscopy. Next, we performed RNA isolation, amplification, labeling and hybridization against 44k Agilent microarrays.

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Expression data from non-pigmented and pigmented mouse melanocytes

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