Project description:gnp3_tri33-arabidoseed. Embryo seed development. WP3: Biodiversity of seed traits: state-of-the-art. Analysis of the expression of small RNAs precursors in the seed development of Arabidopsis thaliana.
Project description:gnp3_tri33-arabidoseed. Embryo seed development. WP3: Biodiversity of seed traits: state-of-the-art. Analysis of the expression of small RNAs precursors in the seed development of Arabidopsis thaliana. 12 dye-swaps. Time course.
Project description:gnp3_tri33-arabidoseed - eqtl analysis (random pair design) - WP3 : Biodiversity of seed traits : state of the art - Comparison of the developping seed transcriptome in 160 Bay0/Sha Recombinant Inbred Lines (RILs) at 10 days after pollinisation. Keywords: genotype and ecotype comparison
Project description:ARDS-mediated lung transcriptome alterations were identified in forest musk deer. Moreover, multiple transcripts/genes involved in lung development and lung defense responses to bacteria/viruses/fungi in ARDS were filtered out in forest musk deer.
Project description:gnp3_tri33-arabidoseed - seed development - WP3 : Biodiversity of seed traits : state of the art - This experiment is a time course performed to choose the best harvesting point to maximise the number of detectable transcripts (8, 10, 12 and 14 dap). Keywords: time course
Project description:A functional biodiversity microarray (EcoChip) prototype has been developed to facilitate the analysis of fungal communities in environmental samples with broad functional and phylogenetic coverage and to enable the incorporation of nucleic acid sequence data as they become available from large-scale (next generation) sequencing projects. A dual probe set (DPS) was designed to detect a) functional enzyme transcripts at conserved protein sites and b) phylogenetic barcoding transcripts at ITS regions present in precursor rRNA. Deviating from the concept of GeoChip-type microarrays, the presented EcoChip microarray phylogenetic information was obtained using a dedicated set of barcoding microarray probes, whereas functional gene expression was analyzed by conserved domain-specific probes. By unlinking these two target groups, the shortage of broad sequence information of functional enzyme-coding genes in environmental communities became less important. The novel EcoChip microarray could be successfully applied to identify specific degradation activities in environmental samples at considerably high phylogenetic resolution. Reproducible and unbiased microarray signals could be obtained with chemically labeled total RNA preparations, thus avoiding the use of enzymatic labeling steps. ITS precursor rRNA was detected for the first time in a microarray experiment, which confirms the applicability of the EcoChip concept to selectively quantify the transcriptionally active part of fungal communities at high phylogenetic resolution. In addition, the chosen microarray platform facilitates the conducting of experiments with high sample throughput in almost any molecular biology laboratory. In this study, two independent RNA samples from a pine forest soil were labelled and hybridised to a custom-made EcoChip microarray consisting of about 9000 probes targeting expressed fungals genes and about 5000 probes targeting the precursor-rRNA of different fungal lineages
