Project description:The libraries contained in this Experiment come from tissue sub-sections of a 54 year old male's testis tissue section obtained from GTEx. They are stranded PE101 Illumina Hi-Seq RNA-Seq libraries from rRNA-depleted Total RNA > 200 nucleotides in size. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:The libraries contained in this Experiment come from tissue sub-sections of a 37 year old male's testis tissue section obtained from GTEx. They are stranded PE101 Illumina Hi-Seq RNA-Seq libraries from rRNA-depleted Total RNA > 200 nucleotides in size. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:PIWI-interacting RNAs(piRNAs) are required for transposon repression, de novo DNA methylation, epigenetic and post-transcriptional regulation of gene expression in the germline. A mist of piRNA biogenesis is the identification of the 3’-5’ exnuclease that trimms the 3’ end of piRNA intermediates to form mature piRNAs in mammals. Here, we show that mice deficient for PNLDC1, an evolutionarily conserved 3’-5’ exonuclease, accumulate 3’ untrimmed piRNA intermediates of 30~40nt. These 3’ extended piRNA intermediates associate with PIWI proteins, but are impaired for function. Male Pnldc1 knockout mice display LINE1 derepression and are sterile owing to chimeric spermatogenetic arrest at spermatocyte and spermatid stages. Our findings illustrate the conserved role of mouse Pnldc1 in 3’ trimming during piRNA maturation. However, since Pnldc1 knockout in mice creates phenotypes distinct from its reported interactive partner, Tdrkh, the panorama of participants in mammalian piRNA 3’ end maturation may be more complex than previously speculated.
Project description:PIWI-interacting RNAs(piRNAs) are required for transposon repression, de novo DNA methylation, epigenetic and post-transcriptional regulation of gene expression in the germline. A mist of piRNA biogenesis is the identification of the 3’-5’ exnuclease that trimms the 3’ end of piRNA intermediates to form mature piRNAs in mammals.Here, we show that mice deficient for PNLDC1, an evolutionarily conserved 3’-5’ exonuclease, accumulate 3’ untrimmed piRNA intermediates of 30~40nt. These 3’ extended piRNA intermediates associate with PIWI proteins, but are impaired for function. Male Pnldc1 knockout mice display LINE1 derepression and are sterile owing to chimeric spermatogenetic arrest at spermatocyte and spermatid stages. Our findings illustrate the conserved role of mouse Pnldc1 in 3’ trimming during piRNA maturation. However, since Pnldc1 knockout in mice creates phenotypes distinct from its reported interactive partner,Tdrkh,the panorama of participants in mammalian piRNA 3’ end maturation may be more complex than previously speculated .There is the sequence of Pnldc1 Total RNA-seq of P10 Testis.
Project description:Wild-type (C57Bl6) mice underwent monocular enucleation at P14 and were sacrificed 4 days later at P18. Bilateral (non-deprived and deprived) visual cortex was dissected from 1-mm thick coronal sections, and total RNA was extracted from the tissue lysate using Trizol (Gibco-BRL). Keywords: repeat
Project description:Mice used were in a mixed background between 129/SvEv and C57BL/6. They were Rp1 knockout mice (Rp1-/-). Triplicates of RNA samples from Rp1-/- neural retinas for hybridization were collected at P18. Each RNA sample included a pool of neural retinas from 3-4 mice. Retinas were all collected at 1-2 pm of the day. Keywords: repeat sample
Project description:Mice used were in a mixed background between 129/SvEv and C57BL/6. They were wildtype littermates of Rp1-/- mice used in parallel experiments. Triplicates of RNA samples from Rp1+/+ neural retinas for hybridization were collected at P18. Each RNA sample included a pool of neural retinas from 3-4 mice. Retinas were all collected at 1-2 pm of the day. Keywords: repeat sample