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Pseudmonas aeruginosa PAO1 wild-type cultured in MOPS Glycerol compared to MOPS Glycerol Hypoxia (restricted oxygen).

ABSTRACT: Pseudmonas aeruginosa PAO1 wild-type cultured in MOPS Glycerol compared to MOPS Glycerol Hypoxia (restricted oxygen).

PROVIDER: PRJEB34916 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3597223.fastq.gz	Fastqsanger.gz
	ERR3597224.fastq.gz	Fastqsanger.gz
	ERR3597225.fastq.gz	Fastqsanger.gz
	ERR3597226.fastq.gz	Fastqsanger.gz
	ERR3597227.fastq.gz	Fastqsanger.gz

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Pseudmonas aeruginosa PAO1 wild-type cultured in MOPS Glycerol compared to MOPS Glycerol Hypoxia (restricted oxygen).

Project description:Pseudmonas aeruginosa PAO1 wild-type cultured in MOPS Glycerol compared to MOPS Glycerol Hypoxia (restricted oxygen). P. aeruginosa strain PAO1 was grown in 40 ml MOPS with glycerol as sole carbon source (triplicate), 37 °C with shaking (250 rpm) in baffled flasks (500 ml). For the restricted oxygen condition, P. aerugionosa was cultured in the same conditions, except non-baffled flasks were used, and the shaking speed was 80 rpm (gentle aggitation). A thin layer 10 ml of mineral oil was overlaid on top of these cultures to restrict oxygen transfer.

2019-12-02 | E-MTAB-8442 | biostudies-arrayexpress

Pseudomonas aerugionsa PAO1 transcriptome cultured in MOPS Glycerol, MOPS Acetate

Project description:Pseudomonas aerugionsa PAO1 transcriptome cultured in MOPS Glycerol, MOPS Acetate

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Pseudomonas aerugionsa PAO1 transcriptome cultured in MOPS Glycerol and MOPS Acetate

Project description:Drastic alterations in transcripts associated with central carbon metabolism and associatedprocesses have been repeatedly observed a common expression program as P. aeruginosa adapts to the cystic fibrosis lung when compared to standard laboratory conditions. However, we have limited knowledge of how well these transcriptomic changes correlate with actual alterations in metabolic flux; the rate of turnover of molecules through metabolic pathways. We have compared the proteome of Pseudomonas aeruginosa PAO1 during growth on the carbon sources acetate and glycerol.

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Pseudomonas aerugionsa PAO1 transcriptome cultured in MOPS Glycerol, MOPS Acetate

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Pseudmonas aeruginosa PAO1 wild-type and delta prpC cultured on MOPS Succinate and MOPS Succinate + Propionate

Project description:Pseudmonas aeruginosa PAO1 wild-type and delta prpC cultured on MOPS Succinate and MOPS Succinate + Propionate

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Pseudmonas aeruginosa PAO1 wild-type and delta prpC cultured on MOPS Succinate and MOPS Succinate + Propionate

Project description:Pseudomonas aeruginosa strain PAO1 (wild-ype and prpC) was grown in 40 ml MOPS with succinate as the sole carbon source, 37 °C with shaking (250 rpm) in baffled flasks (500 ml). During exponential growth, 500 uM propionate was added to the cultures (H2O added to controls). Samples were harvested during mid-exponential growth. Four conditions, 12 samples total (triplicate). 5 ml of culture was harvested from each sample and added to an equal volume of RNAlater® RNA stabilization solution. Samples were then centrifuged at 4000 rpm for 15 min (4 °C) and the pellets were stored at – 80 °C. RNA was extracted using a RNAeasy Mini-Kit according to the manufacturers instructions. RNA was estimated with a NanoDrop ND-1000 spectrophotometer (NanoDrop technologies Inc, USA) and using agarose gel electrophoresis. Ribosomal RNA (rRNA) was then depleted from each sample (5 µg each) using the bacterial Ribo-Zero rRNA Removal Kit (Illumina). The integrity of the RNA was evaluated using an RNA 6000 Nano LabChip and an Agilent 2100 Bioanalyzer (Agilent Technologies, Germany). Eight indexed, strand-specific cDNA libraries were prepared, and samples were sequenced on an Illumina HiSeq 2000 with a 51-bp single-end read length (GATC Biotech, Germany).

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Project description:Mops condylurus Metagenome

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Grad-seq in Pseudomonas aeruginosa PAO1

Project description:Protein complexome analysis of Pseudomonas aeruginosa PAO1 at OD 0.3 in LB media through 10-40% glycerol gradient fractionation in 21 samples.

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