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Striga hermonthica genome assembly

ABSTRACT: Complete sequence of the obligate, root hemi-parasitic plant Striga hermonthica. We sequenced an individual from an accession of S. hermonthica seeds collected from farmer’s fields in Kibos Kenya. An individual was selected at random from parasites growing on the highly susceptible rice cultivar NERICA 7 in a controlled environment experiment.

PROVIDER: PRJEB35606 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	CACSLK01.dat.gz	Other
	CACSLK01.fasta.gz	Fasta.gz
	CACSLK01.master.dat	Other
	ERR3698240_1.fastq.gz	Fastqsanger.gz

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Striga hermonthica

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Project description:Using rice cultivars Nipponbare, which exhibits resistance to Striga hermonthica (a root parasitic plant that causes devastating loss of yield), and IAC165, which is susceptible, we aim to identify suites of genes underlying susceptibility and resistance to S. hermonthica by profiling changes in gene expression using rice whole genome microarrays. In addition to a functional categorisation of changes in gene expression, genes that were significantly differentially regulated within regions predicted to contain Nipponbare quantitative trait loci for resistance were identified. Experiment Overall Design: Root tissue was harvested from two cultivars of rice that had been infected with Striga hermonthica (or from uninfected control tissue). Three timepoints were used (2, 4 and 11 days after infection or mock-inoculation) using two replicate chips per treatment/timepoint.

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