Project description:To test whether non-coding RNAs play roles in regulating response to powdery mildew infection and heat stress in wheat, by using Solexa high-throughput sequencing and computational analysis and experimental approach we cloned the small RNAs and identified 125 putative long npcRNAs from wheat leaves infected by preponderant physiological strain Erysiphe graminis f. sp. tritici (Egt) or by heat stress treatment. Among long non-coding RNAs, some were precursors of small RNAs such as microRNAs and siRNAs, two long npcRNAs were identified as signal recognition particle (SRP) 7S RNA variants, and three were characterized as U3 snoRNAs. Wheat long npcRNAs showed tissue dependent expression patterns and were responsive to powdery mildew infection and heat stress.
Project description:To test whether non-coding RNAs play roles in regulating response to powdery mildew infection and heat stress in wheat, by using Solexa high-throughput sequencing and computational analysis and experimental approach we cloned the small RNAs and identified 125 putative long npcRNAs from wheat leaves infected by preponderant physiological strain Erysiphe graminis f. sp. tritici (Egt) or by heat stress treatment. Among long non-coding RNAs, some were precursors of small RNAs such as microRNAs and siRNAs, two long npcRNAs were identified as signal recognition particle (SRP) 7S RNA variants, and three were characterized as U3 snoRNAs. Wheat long npcRNAs showed tissue dependent expression patterns and were responsive to powdery mildew infection and heat stress. Examination non-coding RNAs of 2 near isogenic lines 8866 (Susceptible) and Pm30 (Resistant) in response to powdery milew and two genotypes CK (insensitive) and TAM107 (insensitive) to heat. CK and TAM107 represent the same material in different treatments (no heat stress or 1hour after heat stress).
Project description:We used two wheat genotypes, the susceptible wheat cultivar ‘8866 ’(S) and its near isogenic line with single powdery mildew resistance gene ‘pm30’ (R), to investigate gene expression changes in response to powdery mildew infection by using Wheat Genome Array
Project description:We used two wheat genotypes, the susceptible wheat cultivar ‘8866 ’(S) and its near isogenic line with single powdery mildew resistance gene ‘pm30’ (R), to investigate gene expression changes in response to powdery mildew infection by using Wheat Genome Array wheat young leveas of near isogenic lines before or 12 hours after powdery mildew infection were selected for RNA extraction and hybridization on Affymetrix microarrays.The leaf samples were harvested from three independent biological replicates, and the leaves without inoculation were regarded as control.
Project description:Puccinia graminis f.sp. tritici (Pgt), the causal agent of stem rust disease in wheat, is one of the most destructive pathogens and can cause severe yield losses. Here, we utilize Hi-C sequencing technology to scaffold and phase the haplotypes for the genome assembly of a US Pgt isolate 99KS76A-1.
Project description:Transgenic wheat plants of cv. Bobwhite were generated that constitutively overexpress class III peroxidase cDNA TaPrx103 (previously TaPERO) under the control of the epidermis-specific GstA1 promoter. Several transgenic lines show enhanced pathogen resistance. In order to test the concept of substantial equivalence, wildtype and transgenic lines were subjected to pathogen attack by Blumeria graminis f.sp. tritici, followed by transcript profiling using the barleyPGRC1 cDNA array.
Project description:We performed RNA-sequencing of Golovinomyces orontii-infected Arabidopsis leaves of wild type, the double or triple mutants of AtMLKLs to examine the role of AtMLKLs in response to the powdery mildew fungus.
Project description:Powdery mildew (PM) is one of the most important and widespread plant diseases caused by obligate biotrophic Ascomycete fungi in the order of Erysiphales. Monocot PM fungi such as Blumeria graminis f.sp. hordei (Bgh) infectious on barley and B. graminis f.sp. tritici (Bgt) infectious on wheat exhibit high-level of host-specialization. By contrast, many dicot PM fungi display rather broad host ranges. To understand why different PM fungi adopt distinct modes of host-adaption, we sequenced the genomes of four dicot PM strains belonging to Golovinomyces cichoracearum (GcC1, GcM1, GcM3) or Oidium neolycopersici (OnM2) and conducted comparative sequence analyses. PM fungi have highly repetitive genomes that are difficult to perform gene prediction. By combing RNA-seq expression evidence with ab initio gene prediction, we successfully improved the number of predicted genes from 4000 to 6000. By comparing the transcriptional profiling from haustoria with mycelia in OnM2 and GcM3, we found that 86%-96% of the predicted genes are expressed in mycelia and/or haustoria, indicating an efficient expression system of PM fungi. Besides, our results showed that gene regulation mechanisms in haustorial cells maybe under gone a much higher level of diversification between OnM2 and GcM3, since they share only a small proportion (21%) of genes up-regulated in huastoria cells. Notably, a higher proportion of candidate effector genes are selectively up-regulated in haustorial cells, agreeing with their function in suppressing host defense and facilitating nutrient uptake.