Project description:In our previous studies, we found that the bursae of Fabricius in chickens infected with IBDV were severely atrophied and resulted in immune suppression in the chickens. Therefore, we decided to detect the differential expression of miRNA in chickens infected with IBDV by RNA-seq. We then used the data obtained from bursa of Fabricius RNA-seq for gene expression profiling. Our ultimate goal is to study the regulatory mechanisms of host mirnas on IBDV viruses or cells.
Project description:Here we present the first characterisation of small RNAs in honey bee reproductive tissues. We conclude that small RNAs are likely to play an integral role in honey bee gametogenesis and reproduction and provide a plausible mechanism for parent-of origin-effects on gene expression and reproductive physiology. present in honey bee reproductive tissues: ovaries, spermatheca, semen, fertilised and unfertilised eggs, and testes.
Project description:Duck reovirus (DRV) is well-studied aquatic bird virus belonging to the Orthoreovirus genus of the Reoviridae family. The bursa of Fabricius is an immunologically organ against virus invasion. However, the responses of the bursa of Fabricius of Cairna moschata to DRV infection are largely unknown. To investigate the immune responses, the proteomes from the control and two DRV strain infected samples (NH and DJ) were compared. In total, 7075 protein were identified, of which 5625 protein were quantified. A number of differentially expressed proteins (DEPs), including 210 DEPs under the HN10 infection and 55 DEPs under the JD10 infection, were identified. Protein network analysis showed that the DEPs enriched in the serine protease system and the innate immune response clusters. For the serine protease systems, coagulation factor IX, three chains of fibrinogen, and complement C8, C5, and C2s were significantly up-regulated, suggesting that the serine protease-mediated immune might be involved in the responses to the HN10 infection. For the innate and adaptive immune system, RIG-I, MDA5, MAPK20, and IRF3 were significantly up-regulated, indicating their important role in the reorganization of invaded virus. Furthermore, the DEPs among different visceral organs (liver, spleen, and the bursa of Fabricius) were compared. coagulation factor IX was significantly up-regulated in the bursa of fabricius, not in the liver and spleen samples, suggesting an important role of the bursa of fabricius in antivirus. Our data may give a comprehensive resource for investigating the regulation mechanism involved in the responses of the bursa of Fabricius of duck to the DRV infections.
Project description:Social caste determination in the honey bee is assumed to be determined by the dietary status of the young larvae and translated into physiological and epigenetic changes through nutrient-sensing pathways. We have employed microRNA gene-microarray, and observed that both worker jelly and royal jelly showed dynamic changes in miRNA content during the 4th to 6th day of larval development . Adding specific miRNAs to royal jelly elicited significant changes in queen larval mRNA expression and in morphological characters of the emerging adult queen bee. We propose that miRNAs in the nurse bee secretions constitute an additional element in the regulatory control of caste determination in the honey bee.
Project description:Social caste determination in the honey bee is assumed to be determined by the dietary status of the young larvae and translated into physiological and epigenetic changes through nutrient-sensing pathways. We have employed Illumina/Solexa sequencing to examine the small RNA content in the bee larval food source, and show that worker jelly is enriched in miRNA complexity and abundance relative to royal jelly. The miRNA levels in worker jelly were 7-215 fold higher than in royal jelly, and both jellies showed dynamic changes in miRNA content during the 4th to 6th day of larval development. Adding specific miRNAs to royal jelly elicited significant changes in queen larval mRNA expression and in morphological characters of the emerging adult queen bee. We propose that miRNAs in the nurse bee secretions constitute an additional element in the regulatory control of caste determination in the honey bee.