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Removal of anionic surfactant in laundry wastewater using EGSB reactor: effect of biomass adaptation

ABSTRACT: For evaluated of biomass adaptation, two EGSB reactors were operated. RAB (adapted biomass) reactor was operated in two stages: Stage I, with standard Linear Alklybenzene Sulfonate - LAS (technical grade; 13.2 mg.L-1); Stage II, the standard LAS, was replaced by diluted laundry wastewater according to LAS concentration (11.2 mg.L-1). RNAB (not adapted biomass) reactor had single stage (Stage I), directly with the wastewater (11.5 mgLAS.L-1). Thus, the 16S rRNA pyrosequencing was performed for two samples from sludge blanket: (i) Stage II - RAB and (ii)Stage I - RNAB. The samples were sequenced in a 454 platform. The two samples were sequenced in a run using barcodes. One sff file contain the reads from all samples.

PROVIDER: PRJEB4790 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR356948.fastq.gz	Fastqsanger.gz
	ERR356949.fastq.gz	Fastqsanger.gz

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Project description:Two-stage two-phase biogas reactor systems consisting each of one batch downflow hydrolysis reactor (HR, vol. 10 L), one process fluid storage tank (vol. 10 L), and one downstream upflow anaerobic filter reactor (AF, vol. 10 L), were operated at mesophilic (M, 37 °C) and thermophilic (T, 55 °C) temperatures and over a period of > 750 d (Figure 1, Additional file 1). For each reactor system and for each process temperature, two replicates were conducted in parallel, denominated further as biological replicates. Further process details were as previously published. Start-up of all fermenters were performed using liquid fermenter material from a biogas plant converting cattle manure in co-digestion with grass and maize silage and other biomass at varying concentrations and at mesophilic temperatures. Silage of perennial ryegrass (Lolium perenne L.) was digested as sole substrate in batches of varying amounts with retention times of 28 d (storage of bale silage at -20 °C, cutting length 3 cm, volatile substances (VS) 32 % of fresh mass (FM), total Kjeldahl nitrogen 7.6 g kgFM-1, NH4+-N 0.7 g kgFM-1, acetic acid 2.6 g kgFM-1, propionic acid < 0.04 g kgFM-1, lactic acid 2.6 g kgFM-1, ethanol 2.2 g kgFM-1, C/N ratio 19.3, chemical oxygen demand (COD) 357.7 g kgFM-1, analysis of chemical properties according to [6]. No spoilage was observed in the silage. Biogas yields were calculated as liters normalized to 0 °C and 1013 hPa (LN) per kilogram volatile substances (kgVS). For chemical analysis, samples were taken from the effluents of HR and AF. For sequencing of 16S rRNA gene amplicon libraries, microbial metagenomes, and microbial metatranscriptomes, samples were taken from the silage digestate in the HR digested for 2 d. At this time point, high AD rates were detected as indicated by the fast increase of volatile fatty acids (VFA), e.g., acetic acid. Sampling was performed at two different organic loading rates (OLR), i.e., batch-fermentation of 500 g (denominated as “low OLR”, samples MOLR500 and TOLR500) and 1,500 g silage (denominated as “increased OLR”, samples MOLR1500 and TOLR1500).

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Removal of anionic surfactant in laundry wastewater using EGSB reactor: effect of biomass adaptation

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