Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

NTRAS hnRNPL RNA sequencing in HUVECs

ABSTRACT: NTRAS hnRNPL RNA sequencing in HUVECs

PROVIDER: PRJEB49865 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR7925572.fastq.gz	Fastqsanger.gz
	ERR7925573.fastq.gz	Fastqsanger.gz
	ERR7925574.fastq.gz	Fastqsanger.gz
	ERR7925575.fastq.gz	Fastqsanger.gz
	ERR7925576.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

NTRAS hnRNPL RNA sequencing in HUVECs

Project description:In the current project with aim to unequivocally characterize a novel splicing-regulatory network that proves to be a central mediator of endothelial barrier function and vascular integrity. At the core of this network is the endothelial enriched lncRNA NTRAS (annotated as RP11-354k1.1) is shown to control alternative splicing decisions in HUVECs through interplaying with splicing factor hnRNPL. Specifically, in the project we show that NTRAS sequesters the splicing factor hnRNPL through a CA dinucleotide motif, to enhance TJP1 exon 20 usage, thereby TJP1α+ isoform. In turn disrupting TJP1α+ isoform expression impaired endothelial barrier function. Collectively, this splicing-regulatory network might prove fundamental in unlocking new interventions strategic to prevent or reverse vascular leakage.

2022-01-10 | E-MTAB-11311 | biostudies-arrayexpress

RNA sequencing of Opa1-silenced HUVECs.

Project description:RNA sequencing of Opa1-silenced HUVECs.

| PRJNA503252 | ENA

RNA sequencing for HUVECs treated with si-FLRT2

Project description:RNA sequencing for HUVECs treated with si-control or si-FLRT2

2022-01-22 | GSE179836 | GEO

HNRNPL RIP-seq in LNCaP prostate cancer cells

Project description:RNA immunoprecipitation coupled with next generation sequencing (RIP-seq) was used to map the HNRNPL-RNA interactome in LNCaP cells.

2016-12-28 | GSE72841 | GEO

Regulartory effect of HNRNPL and LARP on circular RNA expression in LNCaP prostate cancer cells

Project description:Analysis of circular RNA expression in LNCaP prostate cancer cells treated with different siRNAs to define the regulatory effect of HNRNPL and LARP on circular RNA expression.

2017-09-28 | GSE72840 | GEO

RNA sequencing for HUVECs treated with si-FLRT2

Project description:RNA sequencing for HUVECs treated with si-FLRT2

| PRJNA745197 | ENA

Transcriptome sequencing of HUVECs

Project description:Transcriptome sequencing of HUVECs

| PRJNA649251 | ENA

Transcriptome sequencing of HUVECs

Project description:We analyzed the global effect of SOX9 overexpression by sequencing the transcriptomes of HUVECs transduced with SOX9 expression plasmid or an empty vector control.

2022-06-15 | GSE155290 | GEO

Regulartory effect of HNRNPL and LARP on RNA expression in LNCaP prostate cancer cells

Project description:Analysis of RNA expression in LNCaP prostate cancer cells treated with different siRNAs to define the regulatory effect of HNRNPL and LARP on RNA expression.

2017-09-28 | GSE72842 | GEO

Conserved role of hnRNPL in alternative splicing of epigenetic modifiers enables B cell activation

Project description:The multifunctional RNA-binding protein hnRNPL has been implicated in antibody class switching but its broader function in B cells is unknown. Here, we show that hnRNPL is essential for B cell activation, and thereby germinal center and antibody responses. Upon activation, hnRNPL-deficient B cells show proliferation defects and increased apoptosis. The comparative analysis of RNA-seq data from B cells and another 8 hnRNPL-depleted cell types reveals a common function in the Myc and E2F transcriptional programs required for proliferation, likely borne out of a large alternative splicing change affecting multiple transcription regulators. Notably, while individual gene expression changes were cell type specific, several alternative splicing events affecting histone modifiers like SIRT1, KDM6A, and NSD2, were conserved across cell types, which could contribute to gene expression changes upon hnRNPL loss. SIRT1 reduction due to alternative splicing, and other transcriptional changes suggested mitochondrial defects in hnRNPL-deficient B cells. We confirmed dysfunctional mitochondria and ROS overproduction, which could explain the B cell activation defect. Thus, hnRNPL is essential for the resting to activated B cell transition by regulating transcriptional programs, most likely by splicing regulation affecting several histone modifiers.

2024-04-24 | GSE242069 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data