Similar Datasets
Project description:Anatartic microalgae isolation
| PRJEB53368 | ENA
Project description:Sea ice microbial communities produce large amounts of the sulfur metabolite dimethylsulfoniopropionate (DMSP), a precursor of the climate cooling gas dimethylsulfide. Despite their importance to the polar sulfur cycle, drivers and metabolic pathways of sea ice DMSP are uncertain. Here we report the first measurements of sea ice DMSP sulfur isotopic composition (34S/32S ratio, ?34S). ?34S values in ice cores from the Ross Sea and Weddell Sea reveal considerable variability across seasons and between ice horizons (from +10.6 to +23.6‰). We discuss how the most extreme ?34S values observed could be related to unique DMSP cycling in the seasonally extreme physiochemical conditions of isolated brine inclusions in winter-spring. Using cell cultures, we show that part of the DMSP ?34S variability could be explained by distinct DMSP metabolism in sea ice microalgae. These findings advance our understanding of the sea ice sulfur cycle and metabolic adaptations of microbes in extreme environments.
| S-EPMC6279776 | biostudies-literature
Project description:Using RNAseq of small RNA libraries isolated from the gill tissue of the Antarctic fish Trematomus bernacchii we have characterized the termal sensitivity of miRNA homologues in these highly stenothermic fish.
2019-04-02 | GSE128132 | GEO
Project description:Alga-derived lipids represent an attractive potential source of biofuels. However, lipid accumulation in algae is a stress response tightly coupled to growth arrest, thereby imposing a major limitation on productivity. To identify master regulators of lipid accumulation and decipher the regulation of lipid biosynthetic pathway, we performed an integrative chromatin signature and transcriptomic analysis in the alga Chlamydomonas reinhardtii. Genome-wide histone modification profiling revealed remarkable differences in functional chromatin states between algae and higher eukaryotes and uncovered regulatory components at the core of lipid accumulation pathways. We identified the transcription factor PSR1 as a pivotal master switch that triggers cytosolic lipid hyper-accumulation an order of magnitude higher than stress regimens have achieved. Dissection of the PSR1 target network corroborates its central role in coordinating multiple stress responses. The comprehensive maps of functional chromatin signatures in a major clade of eukaryotic life and the discovery of a central regulator of algal lipid metabolism will facilitate targeted engineering strategies in microalgae. 1. Genome-wide H3K4me3 time series profiling (at 0 hr, 10 min, 30 min, 1 hr, 2hr, 6 hr, 8 hr, 24 hr and 48 hr after nitrogen starvation) was performed to determine time point to capture maximal chromatin changes. 2. Genome-wide H3K4me3, H3K27ac, H3K9me3, H3K27me3, H3K36me3 and Pol II profiling were performed at 0 hr, 1 hr after nitrogen starvation and 1 hr after sulfur starvation to determine chromatin signatures. Genome-wide H3K4me2 profiling was performed at 0 hr before starvation. 3. Transcriptome time series profiling (at 0 hr, 10 min, 30 min, 1 hr, 2hr, 6 hr, 8 hr, 24 hr and 48 hr after nitrogen and sulfur starvation separately) for chromatin signature characterization and integrative analysis. 4. Genome-wide PSR1 binding profiling was performed with polyclonal antibody against PSR1 peptide A region and PSR1 peptide B region individually. (At 30 min and 1 hr after nitrogen starvation, and 1 hr, 2 hr and 6 hr after sulfur starvation.) Please note that the following reference genome and gene models used in these experiments are linked below; C.reinhardtii_v5.3_genomic_scaffold_plastids.fasta.gz reference_gene_model.gtf.gz These are based off Phytozome (http://www.phytozome.net/) which does not provide access to earlier version data.
2015-07-28 | E-GEOD-59629 | biostudies-arrayexpress
Project description:Transcriptome analysis of Oleaginous microalgae from the Peruvian Amazon of the genus Ankistrodesmus, Chlorella, and Scenedesmus Transcriptome
| PRJNA361336 | ENA
Project description:Microbiome of different microalgae stock cultures and during a batch growth experiment
| PRJEB46865 | ENA