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Widespread exon-skipping triggers degradation by nuclear RNA surveillance in fission yeast

ABSTRACT: Interrogation of the S. pombe transcriptome using strand specific RNA sequencing (polyA enriched,ribosomal depleted total RNA). A total of 116 transcriptomes were interrogated in the study with the aim to identify exon-skipping events. Expression level summaries were not computed. Only exon-exon junction and exon-skipping diagnostic reads were used throughout. The vast majority (110/116) of transcriptomes are published and available elsewhere, while only 6 transcriptomes were sequenced here: two biological repeats of dhp1-ts and prp2-ts, a single wild-type strain and a single transcription compromised strain - nmt1-rpb4. Note that growth and library construction protocols varied across the samples.

PROVIDER: PRJEB7379 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR637354.fastq.gz	Fastqsanger.gz
	ERR637355.fastq.gz	Fastqsanger.gz
	ERR637356.fastq.gz	Fastqsanger.gz
	ERR637357.fastq.gz	Fastqsanger.gz
	ERR637358.fastq.gz	Fastqsanger.gz

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Cellular senescence induced by down-regulation of PTBP1 correlates with exon skipping of mitochondrial related gene NDUFV3

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