GS112 Indian Ocean
Ontology highlight
ABSTRACT:
REPOSITORIES: ENA
Dataset's files
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| ERR650528.fastq.gz | Fastqsanger.gz |
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Project description:To elucidate how does the gene expression profiles of whole transcriptome of P.fucata response to seawater acidification and warming, we have employed microarray as a tool to identify gene responses in these stress.The pearl oysters were added into the tanks with the maintaining conditions of temperature 25 °C and 31 °C, acidity pH7.8 and pH7.5, salinity 33‰ in recirculating seawater. The temperatures and pH values in the studies were near future levels and predicted for 2100 and 2300.
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Project description:Under global warming, climate models show an almost three-fold increase in extreme positive Indian Ocean Dipole (pIOD) events by 2100. These extreme pIODs are characterised by a westward extension of cold sea surface temperature anomalies (SSTAs) which push the downstream atmospheric convergence further west. This induces severe drought and flooding in the surrounding countries, but the processes involved in this projected increase have not been fully examined. Here we conduct a detailed heat budget analysis of 19 models from phase 5 of the Coupled Model Intercomparison Project and show that nonlinear zonal and vertical heat advection are important for reinforcing extreme pIODs. Under greenhouse warming, these nonlinear processes do not change significantly in amplitude, but the frequency of occurrences surpassing a threshold increases. This is due to the projected weakening of the Walker circulation, which leads to the western tropical Indian Ocean warming faster than the east. As such, the magnitude of SSTAs required to shift convection westward is relatively smaller, allowing these convection shifts to occur more frequently in the future. The associated changes in wind and ocean current anomalies support the zonal and vertical advection terms in a positive feedback process and consequently, moderate pIODs become more extreme-like.
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Project description:The characterization of global marine microbial taxonomic and functional diversity is a primary goal of the Global Ocean Sampling Expedition. As part of this study, 19 water samples were collected aboard the Sorcerer II sailing vessel from the southern Indian Ocean in an effort to more thoroughly understand the lifestyle strategies of the microbial inhabitants of this ultra-oligotrophic region. No investigations of whole virioplankton assemblages have been conducted on waters collected from the Indian Ocean or across multiple size fractions thus far. Therefore, the goals of this study were to examine the effect of size fractionation on viral consortia structure and function and understand the diversity and functional potential of the Indian Ocean virome. Five samples were selected for comprehensive metagenomic exploration; and sequencing was performed on the microbes captured on 3.0-, 0.8- and 0.1 µm membrane filters as well as the viral fraction (<0.1 µm). Phylogenetic approaches were also used to identify predicted proteins of viral origin in the larger fractions of data from all Indian Ocean samples, which were included in subsequent metagenomic analyses. Taxonomic profiling of viral sequences suggested that size fractionation of marine microbial communities enriches for specific groups of viruses within the different size classes and functional characterization further substantiated this observation. Functional analyses also revealed a relative enrichment for metabolic proteins of viral origin that potentially reflect the physiological condition of host cells in the Indian Ocean including those involved in nitrogen metabolism and oxidative phosphorylation. A novel classification method, MGTAXA, was used to assess virus-host relationships in the Indian Ocean by predicting the taxonomy of putative host genera, with Prochlorococcus, Acanthochlois and members of the SAR86 cluster comprising the most abundant predictions. This is the first study to holistically explore virioplankton dynamics across multiple size classes and provides unprecedented insight into virus diversity, metabolic potential and virus-host interactions.
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