Homo sapiens
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ABSTRACT:
PROVIDER: PRJNA104323 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:This SuperSeries is composed of the following subset Series: GSE4919: Translation State Array Analysis of Thrombin Stimulated Human Endothelial Cells GSE5352: Translation State Array Analysis of LPS Stimulated Human Endothelial Cells GSE5353: Translation State Array Analysis of Insulin Stimulated Human Endothelial Cells GSE5354: Translation State Array Analysis of TNF Stimulated Human Endothelial Cells GSE5385: Translation State Array Analysis of Histamine Stimulated Human Endothelial Cells GSE5386: Translation State Array Analysis of Serum Stimulated Human Endothelial Cells Refer to individual Series
2010-07-01 | E-GEOD-5393 | biostudies-arrayexpress
Project description:Translation State Array Analysis of Insulin Stimulated Human Endothelial Cells
| PRJNA104315 | ENA
Project description:Translation State Array Analysis of Thrombin Stimulated Human Endothelial Cells
| PRJNA104455 | ENA
Project description:Translation State Array Analysis of TNF Stimulated Human Endothelial Cells
| PRJNA104317 | ENA
Project description:Translation State Array Analysis of Histamine Stimulated Human Endothelial Cells
| PRJNA104321 | ENA
Project description:Translation State Array Analysis of LPS Stimulated Human Endothelial Cells
| PRJNA104313 | ENA
Project description:Confluent human umbilical vein endothelial cells (HUVECs) were exposed to histamine (100 mM) for 2 hours. Ribosomal profiling via gradient centrifugation and fractionation was used to separate monosome, or under-translated, and polysome, or actively translated, mRNA species that were then used to probe cDNA arrays, a process known as Translation State Array Analysis (TSAA). Four samples were obtained from these experiments, control monosome, control polysome, histamine monosome and histamine polysome. Using the normalized signal intensities from the GeneFilters, we calculated a translation index, or measure of movement of an mRNA molecule from the monosome to the polysome fraction upon stimulation. This calculation was made as follows: (histamine polysome/histamine monosome)/(control polysome/control monosome). Translational indices greater than 2.5 (upregulated) or lower than 0.4 (downregulated) were chosen for further study. Keywords: Translation State Array Analysis
2006-07-27 | GSE5385 | GEO
Project description:Confluent human umbilical vein endothelial cells (HUVECs) were exposed to histamine (100 mM) for 2 hours. Ribosomal profiling via gradient centrifugation and fractionation was used to separate monosome, or under-translated, and polysome, or actively translated, mRNA species that were then used to probe cDNA arrays, a process known as Translation State Array Analysis (TSAA). Four samples were obtained from these experiments, control monosome, control polysome, histamine monosome and histamine polysome. Using the normalized signal intensities from the GeneFilters, we calculated a translation index, or measure of movement of an mRNA molecule from the monosome to the polysome fraction upon stimulation. This calculation was made as follows: (histamine polysome/histamine monosome)/(control polysome/control monosome). Translational indices greater than 2.5 (upregulated) or lower than 0.4 (downregulated) were chosen for further study. Keywords: Translation State Array Analysis
2006-07-27 | GSE5386 | GEO
Project description:Confluent human umbilical vein endothelial cells (HUVECs) were exposed to Insulin (100 micM) for 2 hours. Ribosomal profiling via gradient centrifugation and fractionation was used to separate monosome, or under-translated, and polysome, or actively-translated, mRNA species that were then used to probe cDNA arrays, a process known as Translation State Array Analysis (TSAA). Four samples were obtained from these experiments: control monosome, control polysome, Insulin monosome and Insulin polysome. using the normalized signal intensities from the GeneFilters, we calculated a translation index, or measure of movement of an mRNA molecule from the monosome to the polysome fraction upon stimulation. This calculation was made as follows: (Insulin polysome/Insulin monosome)/(control polysome/control monosome). Translational indices greater than 2.5 (upregulated) or lower than 0.4 (downregulated) were chosen for further study. Keywords: Translation State Array Analysis
2006-07-27 | GSE5353 | GEO