Haematobia irritans
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA109219 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:This SuperSeries is composed of the following subset Series: GSE11363: Female adult hornfly gene expression vs male adult hornfly gene expression GSE11364: Hornfly 1st instar larvae vs pooled adult + egg Refer to individual Series
2010-05-26 | E-GEOD-11828 | biostudies-arrayexpress
Project description:Female adult hornfly gene expression vs male adult hornfly gene expression
| PRJNA109217 | ENA
Project description:Analysis of expression in pof mutant and wt 1st instar larvae Keywords: Mutant vs Wt expression
2007-03-22 | GSE5984 | GEO
Project description:Background: The horn fly, Haematobia irritans (L.), is an obligate blood-feeding parasite of cattle and control of this pest is a continuing problem in the United States and other parts of the world. Worldwide annual economic losses attributable to this pest surpass $1 billion. The fly is becoming resistant to pesticides and new control technologies are needed by cattle producers. Results: Dominant conditional lethal gene systems are being investigated as population control technologies against agricultural insect pests. One of the critical components of these systems is a highly expressed female-specific gene promoter which can be used to drive expression of a lethality-inducing gene. To identify candidate genes to supply this gene promoter, microarrays were designed from a recently developed horn fly EST database and probed to identify female-specific and larval-specific differential gene expression. Analysis of dyeswap experiments found 432 and 417 transcripts which were over- and under-expressed in adult female flies, respectively, compared to adult male flies. Additionally, 419 and 871 transcripts were over- and under-expressed in first instar larvae compared to adult flies. Three transcripts were identified which were over-expressed in adult females flies compared to adult males and which also were over-expressed in the first instar larval lifestage compared to adult flies. Conclusion: We have identified 3 strong candidates for further evaluation as a gene promoter source for the development of a female-specific conditional lethality system in the horn fly. One of these candidates, the putative nanos orthologue, has a high female-to-male expression ratio, has a moderate expression level in first instar larvae, and has been well characterized in D. melanogaster. Further investigations leading from this microarray analysis will include transformation of the horn fly and evaluation of the female conditional lethal system components for applicability to the specific biological parameters of natural populations of the horn fly. Keywords: evaluation of 1st instar larvae genes vs pooled adult + egg genes
2008-06-19 | GSE11364 | GEO
Project description:Investigation of whole genome transcription expression level changes in Drosophila mojavensis wild-type populations (Las Bocas:LB and Punta Prieta:PP). The experiment was designed to investigate life history transcriptomics in different environments. A total of 172 hybridizations were performed in this entire experiment. We used 135K 12-plex NimbleGen arrays. Total RNA was recovered from each sample listed below. The experimental design consisted a total of two populations (Las Bocas:LB; Punta Prieta:PP), two host diets (Agria:AG and Organ pipe:OP) and fourteen life stages (6 hr egg:E6; 1st instar larvae:L1; 2nd instar larvae:L2; 3rd instar larvae:L3; Early pupal stage:EP; Late pupal stage:LP; 0 Day old adult:0D; 3 Day old adult:3D; 4 Day old adult: 4D; 6 Day old adult:6D; 10 Day old adult:10D; 14 Day old adult:14D; 18 Day old adult:18D & 24 Day old adult:24D). Each chip measures the expression level of 14528 transcripts. Two to 5 replicates were used for each type (R1, R2, R3 etc.). Fly source details are as follows: Las Bocas 2009: LB09; Punta Prieta 2008:PP08.
2014-01-30 | E-GEOD-54510 | biostudies-arrayexpress
Project description:Transcriptional analysis of sex-regulated genes from male and female dsxM-^VeGFP sexed larvae (1st instar, late 2nd /early 3rd instar and 4th instar), pupae and virgin non-blood-fed 3 day old adult A. gambiae mosquitoes
2011-05-01 | E-MEXP-3093 | biostudies-arrayexpress
Project description:Microarray profiling of GFP-marked epithelial cells isolated by FACS from 1st and 3rd instar wild type and mutant Drosophila larvae.
2014-08-04 | GSE50540 | GEO
Project description:Analysis of expression in pof mutant and wt 1st instar larvae Experiment Overall Design: 400 1st instar larvae were used for each of 4 biological replicates of pof mutants and 3 replicates of wt larvae.
2007-10-25 | E-GEOD-5984 | biostudies-arrayexpress
Project description:The horn fly is an obligate blood-feeding parasite of cattle
| PRJNA30973 | ENA