Trichoderma reesei
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ABSTRACT:
PROVIDER: PRJNA110947 | ENA |
REPOSITORIES: ENA
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Project description:A 1,4-beta-D-glucan cellobiohydrolase (EC 3.2.1.91) was purified from the culture liquid of Trichoderma reesei by using biospecific sorption on amorphous cellulose and immunoaffinity chromatography. A single protein band in polyacrylamide-gel electrophoresis and one arc in immunoelectrophoresis corresponded to the enzyme activity. The Mr was 65 000. The pI was 4.2-3.6. The purified enzyme contained about 10% hexose. The enzyme differs from previously described cellobiohydrolases in being more effective in the hydrolysis of cellulose.
| S-EPMC1152451 | biostudies-other
Project description:BACKGROUND:The tropical ascomycete Trichoderma reesei (Hypocrea jecorina) represents one of the most efficient plant cell wall degraders. Regulation of the enzymes required for this process is affected by nutritional signals as well as other environmental signals including light. RESULTS:Our transcriptome analysis of strains lacking the photoreceptors BLR1 and BLR2 as well as ENV1 revealed a considerable increase in the number of genes showing significantly different transcript levels in light and darkness compared to wild-type. We show that members of all glycoside hydrolase families can be subject to light dependent regulation, hence confirming nutrient utilization including plant cell wall degradation as a major output pathway of light signalling. In contrast to N. crassa, photoreceptor mediated regulation of carbon metabolism in T. reesei occurs primarily by BLR1 and BLR2 via their positive effect on induction of env1 transcription, rather than by a presumed negative effect of ENV1 on the function of the BLR complex. Nevertheless, genes consistently regulated by photoreceptors in N. crassa and T. reesei are significantly enriched in carbon metabolic functions. Hence, different regulatory mechanisms are operative in these two fungi, while the light dependent regulation of plant cell wall degradation appears to be conserved.Analysis of growth on different carbon sources revealed that the oxidoreductive D-galactose and pentose catabolism is influenced by light and ENV1. Transcriptional regulation of the target enzymes in these pathways is enhanced by light and influenced by ENV1, BLR1 and/or BLR2. Additionally we detected an ENV1-regulated genomic cluster of 9 genes including the D-mannitol dehydrogenase gene lxr1, with two genes of this cluster showing consistent regulation in N. crassa. CONCLUSIONS:We show that one major output pathway of light signalling in Trichoderma reesei is regulation of glycoside hydrolase genes and the degradation of hemicellulose building blocks. Targets of ENV1 and BLR1/BLR2 are for the most part distinct and indicate individual functions for ENV1 and the BLR complex besides their postulated regulatory interrelationship.
| S-EPMC3831817 | biostudies-literature
Project description:ArrayCGH of Trichoderma reesei strains QM9123, QM9414, NG14 and RutC-30
| PRJNA122443 | ENA
Project description:The ß-importin KAP8 (Pse1/Kap121) is required for nuclear import of the cellulase transcriptional regulator XYR1, asexual sporulation and stress resistance in Trichoderma reesei
| PRJNA255678 | ENA
Project description:Trichoderma reesei QM6a CGH self hybridization
| PRJNA118357 | ENA
Project description:Decoding the black box of cellulase formation in Hypocrea jecorina by RNA-Seq analysis
| PRJNA232512 | ENA
Project description:Expression analysis of Hypocrea jecorina CBS999.97(MAT1-2) wild-type and delta-env1 strains in four different light and darkness conditions
| PRJNA170416 | ENA
Project description:Expression analysis of protein production in the filamentous fungus Trichoderma reesei (Hypocrea jecorina)
| PRJNA143547 | ENA
Project description:Transcriptome during shift of Trichoderma reesei from mycelial growth to asexual sporulation
| PRJNA138443 | ENA
Project description:To investigate the potential role in mycoparasitism, microarrays were used to examine T. reesei transcript levels when confronted with a potential prey (the plant pathogen Rhizoctonia solani) before contact, during first physical contact and during overgrowth of the host.
2011-07-01 | GSE23410 | GEO