Project description:In the complex lifecycle of schistosomes, four developmental stages are closely associated with their definitive hosts: cercaria (infective stage), schistosomula and adult worm (parasitic stages), egg (pathogenic- and pathophoresis-stage). We have examined the gene expression profiles of Schistosoma japonicum in the four developmental stages. Genes with different expression patterns were identified and the information obtained will help indentify new anti-schistosomal intervention targets in the future.
Project description:It is well recognized that parasitic helminth infections, which afflict more than one billion people globally, correlate with a decreased prevalence of metabolic diseases, including obesity and type 2 diabetes, but the molecular mechanisms involved remain to be determined. Using microarrays, we quantified the temporal gene expression profiles in the liver of Schistosoma japonicum-infected C57BL/6 mice at 9 weeks post infection with that from uninfected mice as controls. More than 150 miRNAs were differentially expressed in the liver during S. japonicum infection, and miRNA-mRNA network would provide new evidence for the negtive correlation between S. japonicum infection and metabolism.
Project description:We report on the small RNA profiles of Schistosoma japonicum (S. japonicum) miRNAs using small RNA deep sequencing in the key stages of male-female pairing, gametogenesis, and egg production.
Project description:We report on the small RNA profiles of Schistosoma japonicum (S. japonicum) miRNAs using small RNA deep sequencing in the key stages of male-female pairing, gametogenesis, and egg production. Examination of different miRNAs between males and females in Schistosoma japonicum
Project description:The gogal of this study is to use RNA-Seq to systematically investigate the dynamics of the liver transcriptome over Schistosoma japonicum infection.
