Project description:To investigate the effects of dcl3,dcl4,mDCL,rdr2,rdr6,nrpd1b, and nrpd1c mutations upon small RNAs in Physcomitrella patens , small RNA-seq was performed.
Project description:To investigate the effects of dcl3,dcl4,mDCL,rdr2,rdr6,nrpd1b, and nrpd1c mutations upon small RNAs in Physcomitrella patens , small RNA-seq was performed. Total RNA samples were obtained from the indicated mutants and subject to small RNA-seq using the Illumina TruSeq Small RNA protocol, and analyzed on a HiSeq2500 instrument
Project description:To investigate the effects of rdr2 and rdr6 mutations upon the strucutred component of the Physcomitrella patens transcriptome, dsRNAseq performed. The accumulation of dsRNAseq reads within known small RNA (microRNA and siRNA) loci was quantified and compared between wild-type and the two mutants.
Project description:To investigate the effects of rdr2 and rdr6 mutations upon small RNAs in Physcomitrella patens , small RNA-seq was performed. Total RNA samples were obtained from wild-type, rdr2, and rdr6, and subject to small RNA-seq via the protocol described by Zheng et al. (2010) (PMID: 20941385)
