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Mus musculus

ABSTRACT: Differentially regulated genes in control and c-myc N-myc deficient LT-HSCs

OTHER RELATED OMICS DATASETS IN: GSE12538

PROVIDER: PRJNA114221 | ENA |

REPOSITORIES: ENA

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Project description:Differentially regulated genes in control and c-myc N-myc deficient LT-HSCs and progenitors

| PRJNA112893 | ENA

Mus musculus

Project description:Differentially regulated genes in control and c-myc N-myc deficient progenitors

| PRJNA114237 | ENA

Mus musculus

Project description:Differentially regulated genes in LT-HSC from control or Pbx1-null mice

| PRJNA105243 | ENA

Mus musculus

Project description:Differentially regulated genes in normal LT-HSC vs ST-HSC

| PRJNA105245 | ENA

Differentially regulated genes in control and c-myc N-myc deficient LT-HSCs

Project description:Analysis of HSCs from control and c-myc N-myc deficient long-term hematopoietic stem cells. HSCs lacking both c-myc and N-myc display increased apoptosis rates. Data provide insight into the molecular changes occuring upon complete loss of Myc activity, clarifying the resulting apoptotic mechanism and the role of Myc family proteins in HSCs. LT-HSC (Lin-Sca1+CD150+CD48-) cells were sorted from the BM of MxCre c-myc flox2 N-myc flox2 (experimental) and c-myc flox2 N-myc flox2 (control) mice 3 days after the last pI-pC injection. Each condition was analysed in triplicates, with each replicate consisting of a pool of 3 dKO mice or 2 control mice.

2009-02-20 | E-GEOD-12467 | biostudies-arrayexpress

Identification of Fam122a regulated genes in LT-HSCs.

Project description:Identification of Fam122a regulated genes in LT-HSCs.

| PRJNA635351 | ENA

LIGHT/LTβR signaling regulates self-renewal and differentiation of hematopoietic and leukemia stem cells

Project description:Purpose: The goal of this study is to compare NGS-derived transcriptome profiling (RNA-seq) of Ltbr-deficient and -proficient LT/ST-HSCs isolated from chimeric mice. Methods: Transcriptomic profiles of Ltbr-/- and Ly5.1 LT/ST-HSCs isolated from chimeric mice 6 weeks after reconstitution were assessed in triplicate by deep sequencing, using Illumina NextSeq 500. qRT–PCR validation was performed using TaqMan and SYBR Green assays. Results: We mapped about 60 million sequence reads per sample to the mouse genome (GRCm38 - mm10) and identified expressed transcripts in Ltbr-/- and Ly5.1 LT/ST-HSCs isolated from chimeric mice. RNA-seq. data confirmed stable expression of known housekeeping genes. Differentially expressed genes between the Ltbr-/- and Ly5.1 LT/ST-HSCs were identified with a fold change ≥1.5 and FDR p-value <0.05. Conclusions: Our study represents the first detailed transcriptome analysis of Ltbr-deficient and -proficient LT/ST-HSCs, with biologic replicates, generated by RNA-seq. technology. Our results show that Ltbr signaling regulates HSC proliferation and differentiation. Evaluation of mRNA content in Ltbr-/- LT/ST-HSCs revealed that Ltbr-deficiency enhances HSC proliferation, differentiation, cell cycle and reduces the activity of canonical NFkB signaling.

2020-10-23 | GSE141206 | GEO

Mus musculus

Project description:Analysis of differentially expressed genes in murine Satb1-deficient hematopoietic stem cells (HSCs) compared to wild-type HSCs

| PRJNA188696 | ENA

Mus musculus

Project description:Expression data from control and LRF (leukemia/lymphoma related factor)-deficient mouse LT-HSCs

| PRJNA178296 | ENA

Differentially regulated genes in control and c-myc N-myc deficient progenitors

2009-02-20 | GSE12536 | GEO