Project description:Microglia activation is a key modulator of brain vulnerability in response to intra-uterine growth restriction (IUGR). However, the consequences of IUGR on microglia development and on microglia proteome are still unknown. We used a model of IUGR induced by a gestational low-protein diet (LPD) in rat. Microglia were isolated from control and LPD animals at P1 and P4. The aim of this work was characterize the consequence of IUGR on microglia development and investigate the consequences of IUGR exposure on microglia proteome during the first postnatal days using a bioinformatics approach associated with functional assays.
Project description:This paper describe the whole genome-wide analysis of differences in postnatal intestinal from 0 day to 21 days of piglets. Using Genome Array,We generated transcriptase profiles of intestine from the Landrace piglets at 0, 3, 8, 14,21 days post natal (dpn). We identified 4014 differentially expressed transcripts during intestinal developing in Landrace piglets. Respectively, the Gene Ontology processes and the time sequence profile analysis of differentially expressed genes were constructed. In the course of growth , the mostly significant enriched GO category of profile mainly focus on folic acid transport, lipopolysaccharide-mediated signaling pathway, Momolybdopterin cofactor biosynthetic process,The down-regulation genes show the state of malnutritionplay at early growth in pig life.
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig 47 samples