Project description:This SuperSeries is composed of the following subset Series: GSE16769: Male and female Fundulus exposed to 172ppb arsenic, and their parentally exposed offspring GSE16770: Male and female Fundulus exposed to 575ppb arsenic, and their parentally exposed offspring GSE16771: Male Fundulus exposed to 1,720ppb arsenic, and their parentally exposed offspring Refer to individual Series
Project description:Cr(III) is the dominant toxicant at some Superfund sites within the United States and therefore we are interested in its effects. Cr(III)’s mechanisms are not well studied or understood because of its low bioavailability. We have attempted to characterize the effects of Cr(III) on gene expression in the liver of adult male Fundulus heteroclitus. The NOEC and LOEC were determined at 32 and 64mg/L, respectively, by measuring growth after exposing juveniles for 30 days. Secondary exposures were performed with adult males at 32mg/L, livers excised, and RNA extracted. Microarrays were probed with cDNA from untreated or Cr(III)-exposed adult fish and gene expression was quantified. Cr(III) at 32mg/L altered the expression of 5 genes, including GSTalpha, GSTtheta, and ALDH4. Ultimately, we anticipate using this gene expression information to determine whether chromium is available at potentially adverse concentrations in contaminated sites. Keywords: dose response
