Project description:multiplexage : dual-target hybridization microarray experiment-New developments towards a genetic and diagnosis use of plant mircoarrays
Project description:Comparing RNA Seq- Data of all three sample types- pathogenic growth, saprophytic growth on same plant substrate, axenic growth under starvation after consumption of the mycotoxin inducing nitrogen source L-ornithine- allows to distinguish between genes whose transcription is affected by nutrient starvation or plant matrix effects from those that respond to defense mechanisms deriving from the active, living plant and thus may allow new insights into pathogenicity of the fungus.
Project description:gnp06-01_agriarray - nitrogen starvation - Several factors affect today a more extended use of microarrays for research and as a diagnosis tool. These factors are the experimental cost, the reproducibility of the measurements and the format of the analyses. This project aims at bringing solutions in these three domains by optimizing multiplexed analyses in order to reduce cost and enhance the number of samples treated simultaneously; by proposing new couples flurophores / slide surface in order to increase the signal precision and by developing new applications such as Comparative Genomic Hybridization (GGH) or Promoter Arrays to bring the plant microarray beyond transcriptome analyses. Altogether, these approaches will allow us to construct an optimized diagnostic tool based on 96 well microplate microarrays. This multidisciplinary project bring together biologists, chemists, physicists and mathematicians to develop innovative solutions for future application of DNA microarrays. - Dual-target hybridization microarray experiment. 2 dye-swap - time course,tissue comparison
Project description:gnp06-01_agriarray - nitrogen starvation - Several factors affect today a more extended use of microarrays for research and as a diagnosis tool. These factors are the experimental cost, the reproducibility of the measurements and the format of the analyses. This project aims at bringing solutions in these three domains by optimizing multiplexed analyses in order to reduce cost and enhance the number of samples treated simultaneously; by proposing new couples flurophores / slide surface in order to increase the signal precision and by developing new applications such as Comparative Genomic Hybridization (GGH) or Promoter Arrays to bring the plant microarray beyond transcriptome analyses. Altogether, these approaches will allow us to construct an optimized diagnostic tool based on 96 well microplate microarrays. This multidisciplinary project bring together biologists, chemists, physicists and mathematicians to develop innovative solutions for future application of DNA microarrays. - Dual-target hybridization microarray experiment.
Project description:Nitrogen and light are two major regulators of plant metabolism and development. While genes involved in the control of each of these signals have begun to be identified, regulators that integrate gene responses to nitrogen and light signals have yet to be determined. Here, we evaluate the role of bZIP1, a transcription factor involved in light and nitrogen sensing, by exposing wild-type (WT) and bZIP1 T-DNA null mutant plants to a combinatorial space of N and L treatment conditions. We use ANOVA analysis combined with clustering and Boolean modeling, to evaluate the role of bZIP1 in mediating L and N signaling genome-wide.
Project description:Comparing RNA Seq- Data of all three sample types- pathogenic growth, saprophytic growth on same plant substrate, axenic growth under starvation after consumption of the mycotoxin inducing nitrogen source L-ornithine- allows to distinguish between genes whose transcription is affected by nutrient starvation or plant matrix effects from those that respond to defense mechanisms deriving from the active, living plant and thus may allow new insights into pathogenicity of the fungus. Three different growth conditions with two to three replicates
Project description:Nitrogen (N) is a key nutrient that is often the limiting factor in plant growth. However, the molecular mechanisms underlying transcriptional regulation of N-starvation-responses remain largely unknown. AtNIGT1s are putative regulators of nitrogen-starvation responsive transcriptome in arabidopsis. We aimed to identify AtNIGT1s target genes by microarray experments.