Project description:MicroRNA profiling of cultured sympathetic neurons from the superior cervical ganglia of E21 Sprague-Dawley rat pups following growth under control conditions or after treatment with BMP-7 at 50 ng/mL for 6 hours or 24 hours.
Project description:We performed RNA-seq on six samples of P20 Sprague Dawley rat superior colliculus, three control rats that underwent eye opening at P14 until P20 and three deprived rats which had their eyes glued closed from P14 until P20. Each sample was created from two pooled colliculi. RNA-seq of the superior colliculus with and without long term light deprivation.
Project description:The rat is a widely used model for human physiology and psychology. However, due to their longer gestation periods and husbandry costs relative to mice, as well as the availability of superior molecular techniques to manipulate the mouse genome, relatively few genome-wide studies have been conducted in the rat. As such, a comprehensive analysis of genomic imprinting, which impacts complex traits such as growth and behaviour, has yet to be conducted in the rat. Towards this end, we systematically profiled embryonic and extra-embryonic tissue gene expression and DNA methylation with allele-specific resolution. We identified 14 embryonic and 26 extra-embryonic imprinted genes in the rat. Comparative analyses with mouse revealed that orthologous imprinted gene expression and associated canonical DNA methylation imprints are conserved in the embryo proper of the Muridae family. However, only 3 paternally expressed imprinted genes in the extra-embryonic embryo are conserved in Muridae, all of which are associated with non-canonical H3K27me3 imprints. The discovery of 8 novel candidate non-canonical imprinted genes unique to the rat is consistent with more rapid evolution of extra-embryonic imprinting. Cross-species analysis of novel rat imprinted genes with mouse and human revealed multiple mechanisms by which species-specific imprinted expression may be established, including H3K27me3 deposition in the oocyte, the birth of ZFP57 binding motifs and the insertion of endogenous retroviral promoters. Thus, characterization of the rat imprintome provides insights into the conservation of imprinted gene expression and the etiology of species-specific imprinting.
2023-01-19 | GSE185573 | GEO
Project description:Transcriptomes of Coregonus artedi complex from Lake Superior
Project description:To study and compare the genes and miRNA expression during the development of the Organ of Corti and the Superior Olivary complex, representing the peripheral and the central auditory systems.
