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Burkholderia glumae

ABSTRACT: RNA-Seq analysis of quorum sensing-dependent genes in Burkholderia glumae BGR1

PROVIDER: PRJNA153523 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR445017.fastq.gz	Fastqsanger.gz
	SRR445018.fastq.gz	Fastqsanger.gz
	SRR445019.fastq.gz	Fastqsanger.gz
	SRR445020.fastq.gz	Fastqsanger.gz
	SRR445021.fastq.gz	Fastqsanger.gz

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RNA-Seq analysis of quorum sensing-dependent genes in Burkholderia glumae BGR1

Project description:Burkholderia glumae causes rice grain rot and sheath rot by producing toxoflavin, whose expression is regulated by quorum sensing (QS). The QS systems of the bacterium rely on N-octanoyl homoserine lactone, synthesized by TofI and its cognate receptor TofR, to activate toxoflavin biosynthesis genes and an IclR-type transcriptional regulator gene, qsmR. To understand genome-wide transcriptional profiling of QS signaling, we employed RNA-Seq of the wild type Burkholderia glumae BGR1 and two QS-defective mutants, BGS2 (BGR1 tofI::Ω) and BGS9 (BGR1 qsmR::Ω), with two different types of culture conditions including 6hr liquid culture (before onset QS) and 10hr liquid culture (after onset QS).

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