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Aspergillus clavatus NRRL 1

ABSTRACT: WGS sequencing, assembly, and annotation

PROVIDER: PRJNA15664 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	AAKD03.dat.gz	Other
	AAKD03.fasta.gz	Fasta.gz
	AAKD03.master.dat	Other
	AAKD03.dat.gz	Other

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Identification and engineering of the cytochalasin gene cluster from Aspergillus clavatus NRRL 1.

Project description:Cytochalasins are a group of fungal secondary metabolites with diverse structures and bioactivities, including cytochalasin E produced by Aspergillus clavatus, which is a potent anti-angiogenic agent. Here, we report the identification and characterization of the cytochalasin gene cluster from A. clavatus NRRL 1. As a producer of cytochalasin E and K, the genome of A. clavatus was analyzed and the ?30 kb ccs gene cluster was identified based on the presence of a polyketide synthase-nonribosomal peptide synthetases (PKS-NRPS) and a putative Baeyer-Villiger monooxygenase (BVMO). Deletion of the central PKS-NRPS gene, ccsA, abolished the production of cytochalasin E and K, confirming the association between the natural products and the gene cluster. Based on bioinformatic analysis, a putative biosynthetic pathway is proposed. Furthermore, overexpression of the pathway specific regulator ccsR elevated the titer of cytochalasin E from 25mg/L to 175 mg/L. Our results not only shed light on the biosynthesis of cytochalasins, but also provided genetic tools for increasing and engineering the production.

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