Coxiella burnetii Dugway 5J108-111
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ABSTRACT:
PROVIDER: PRJNA16721 | ENA |
REPOSITORIES: ENA
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Project description:Nine Mile phase I isolate that causes Q fever
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Project description:Host cell-free growth of the Q fever bacterium Coxiella burnetii
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Project description:Coxiella burnetii, the agent of Q fever, persists in humans despite specific immune responses: however, its reservoir remains unknown. We detected C. burnetii in adipose tissue from BALB/c and C57/BL6 mice 4 months after infection when no bacteria were found in other tissues. C. burnetii infected cultivated adipocytes, replicated within late phagosomes and induced a transcriptional program that was enriched for the expression of genes associated with inflammatory response, hormonal responses and cytoskeleton.
2014-05-25 | GSE46335 | GEO
Project description:The inability to propagate obligate intracellular pathogens under axenic (host cell-free) culture conditions imposes severe experimental constraints that have negatively impacted progress in understanding pathogen virulence and disease mechanisms. Coxiella burnetii, the causative agent of human Q (Query) fever, is an obligate intracellular bacterial pathogen that replicates exclusively in an acidified, lysosome-like vacuole. To define conditions that support C. burnetii growth, we systematically evaluated the organism’s metabolic requirements using expression microarrays, genomic reconstruction, and metabolite typing. This led to development of a complex nutrient medium that supported substantial growth (~ 3 log10) of C. burnetii in a 2.5% oxygen environment. Importantly, axenically grown C. burnetii were highly infectious for Vero cells and exhibited developmental forms characteristic of in vivo grown organisms. Axenic cultivation of C. burnetii will facilitate studies of the organism’s pathogenesis and genetics, and aid development of Q fever preventatives such as an effective subunit vaccine. Furthermore, the systematic approach used here may be broadly applicable to development of axenic media that support growth of other medically important obligate intracellular pathogens.
2009-10-21 | GSE13338 | GEO
Project description:Coxiella burnetii is an intracellular pathogen that causes Q fever, a zoonotic disease found worldwide. In humans, the bacterium is responsible for an acute and chronic disease. It is transmitted to humans by the inhalation of aerosols or contaminated dust containing bacteria from infected livestock. Recently C. burnetii re-emerged in Europe making considerable economic impact in farming industry and even causing multiple human cases. Antibiotic resistance represents a global public health problem; moreover, there are few reports about doxycycline resistance in C. burnetii. Understanding drug application consequences in tolerant bacterial strains is indispensable for the effective therapy. Herein, we report changes in proteome of C. burnetii challenged by antibiotic stress, highlighting polypeptides likely involved in the detoxification of doxycycline. These data were corroborated by gene expression analysis. Based on the identities of differentially abundant proteins we suggest that homeostasis of bacterial surface, oxidative stress defense and balanced carbon metabolism are key processes to survive doxycycline treatment.
2019-08-14 | PXD010250 | Pride
Project description:Mast cells (MCs) are critical mediators of inflammation; however, their microbicidal activity against invading pathogens remains largely unknown. Here, we describe a nonpreviously reported antibacterial mechanism used by MCs against Coxiella burnetii, the agent of Q fever. We show that C. burnetii interaction with MCs does not result in bacterial uptake but rather induces the formation of extracellular actin filaments named cytonemes. MC cytonemes express cathelicidin and neutrophil elastase and mediate the capture and destruction of entrapped bacteria. We provide evidence that MC cytoneme formation and microbicidal activity are dependent on the cooperation of the scavenger receptor CD36 and Toll-like receptor 4. Taken together, our results suggest that MCs use an extracellular sophisticated mechanism of defense to eliminate intracellular pathogens, such as C. burnetii, before their entry into host cells.
2019-03-13 | GSE111971 | GEO