Project description:Global transcriptome profiling of suceptible and tolerant lines of Brassica napus infected with Sclerotinia sclerotiorum using a petal inoculation method that mimics field conditions.
Project description:This SuperSeries is composed of the following subset Series: GSE15337: Gene expression profiling soybean stem tissue early response to Sclerotinia sclerotiorum 1 GSE15338: Gene expression profiling soybean stem tissue early response to Sclerotinia sclerotiorum 3 GSE15339: Gene expression profiling soybean stem tissue early response to Sclerotinia sclerotiorum 4 GSE15340: Gene expression profiling soybean stem tissue early response to Sclerotinia sclerotiorum 2 Refer to individual Series
Project description:RNA-seq of Sclerotinia sclerotiorum-infected B. napus leaves during a timecourse after inoculation. This is a time course experiment. Sclerotinia hyphae were place on detached B. napus leaves, then the combined tissues were harvested at 1, 3, 6, 12, 24, and 48 hours post-innoculation. 3 biological replicates were collected for each time point, and sclerotinia from liquid medium was used as a control.
Project description:Sclerotinia sclerotiorum is a broad-host range necrotrophic pathogen which is the causative agent of Sclerotinia stem rot (SSR), and a major disease of soybean (Glycine max). A time course transcriptomic analysis was performed in both compatible and incompatible soybean lines to identify pathogenicity and developmental factors utilized by S. sclerotiorum to achieve pathogenic success.
Project description:This study provides a first large-scale cloning and characterization of Sclerotinia sclerotiorum milRNAs and milRNAs candidates. Two microRNA-like RNAs (milRNAs) and 42 milRNA candidates were identified by sequence analysis. These milRNAs and candidates provide new insights into the functional roles of small RNAs and adds new resources for the study of plant pathogenic fungi. We constructed a small RNA library from Sclerotinia sclerotiorum.
