Project description:We developed a new method of preparing libraries for strand-specific RNA sequencing (ssRNA-Seq). It employs Direct Ligation of Adaptors to First-strand cDNA (DLAF). We compared ssRNA-Seq libraries prepared using either the DLAF and dUTP methods from mouse embryonic stem cells (mES) and libraries were sequenced from one end or both ends. We also conducted a comparison of ssRNA-Seq libraries prepared using DLAF and ScriptSeq v2 kit (Epicenter) from mouse embryonic cortex (mECx).

2015-01-01 | GSE63424 | GEO

Sequencing of First-strand cDNA Library Reveals Full-length Transcriptomes

2015-01-01 | E-GEOD-63424 | biostudies-arrayexpress

rice ssRNA-seq

Project description:rice ssRNA-seq

| PRJEB2597 | ENA

rice ssRNA-seq

Project description:We took advantage of ssRNA-seq technology to deeply sequence mRNAs of the model plant species Oryza sativa ssp.japonica cv Nipponbare with clear transcriptional orientations for assessing rice cis-NATs at the best possible resolution. We also deeply sequenced rice small RNAs from the same tissues as that for preparing mRNAs to investigate rice cis-NAT pairs that potentially give rise to endogenous short interfering RNAs from their overlapping regions under normal and stress conditions.

2011-12-28 | E-MTAB-721 | biostudies-arrayexpress

unclassified viroids

Project description:unclassified viroids

| PRJNA17012 | ENA