Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparative analysis of Gallus gallus cecal epithelia following Eimeria tenella infection

ABSTRACT: Comparative analysis of Gallus gallus cecal epithelia following Eimeria tenella infection

PROVIDER: PRJNA171222 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Comparative analysis of Gallus gallus cecal epithelia following Eimeria tenella infection

2012-12-10 | GSE39602 | GEO

Comparative analysis of Gallus gallus cecal epithelia following Eimeria tenella infection

Project description:Relative expression levels of mRNAs in chicken cecal epithelia experimentally infected with Eimeria tenella were measured at 4.5 days post-infection. Two weeks old chickens were uninfected (negative control) or were orally inoculated with sporulated oocysts of Eimeria tenella. Cecal epithelia samples were collected from >12 birds in infected or uninfected group at 4.5 d following infections, in which samples from 4 birds were pooled together to form a total 3 biological replicates in each group. Parasite merozoites were also collected from four infected chickens at 5 d after infections. Uninfected control samples, merozoites and infection group samples were selected for RNA extraction and hybridization on Affymetrix microarrays. We used Affymetrix GeneChip chicken genome arrays to detail the chicken cecal epithelia gene expression in the control and E. tenella-infected birds. Infected, uninfected chicken cecal epithelia and merozoites were selected for RNA extraction and hybridization with Affymetrix microarrays. Our goal was to analyze global transcriptome changes in chicken cecal mucous membranes in response to E. tenella infection in vivo. We used infected (T1,T2,T3; three biological replicates) and uninfected (Neg1, Neg2, Neg3; three biological replicates) samples to identify genes that were differentially expressed. Meanwhile, RNA and probes were also prepared from parasite merozoites (Mzt) from infected samples (Mzt) and used as an additional control in microarray hybridization.

2012-12-10 | E-GEOD-39602 | biostudies-arrayexpress

Eimeria tenella cecal mirna expression

Project description:Eimeria tenella cecal mirna expression

| PRJEB48934 | ENA

Eimeria tenella

Project description:Eimeria tenella Genome sequencing

| PRJNA207024 | ENA

Dual RNA-seq transcriptome analysis of chicken caeca infected with Eimeria tenella

Project description:In order to elucidate the infection mechanisms of Eimeria tenella and the chicken immune response, chickens were infected with Eimeria tenella strain Hougton sporozoites. Samples were taken at 0, 1, 2, 3, 4 and 10 days post-infection and mRNA sequenced. A dual-RNA seq analysis was carried out, comparing the expression of infected chickens during each sampling time point with uninfected chickens and comparing E. tenella samples during the infection with a sample of pure sporozoites. The results show a variety of response signals in the chicken, both previously known and unknown, as well as a clear role for a variety of infection-related genes in E. tenella

2021-09-22 | GSE160169 | GEO

Eimeria tenella

Project description:Transcriptome analysis of Eimeria tenella

| PRJDB2533 | ENA

Eimeria tenella genomes

Project description:Eimeria tenella genome sequencing

| PRJEB4009 | ENA

Dual RNA-seq transcriptome analysis of chicken macrophage-like cells (HD11) infected in vitro with Eimeria tenella

Project description:In order to elucidate the infection mechanisms of Eimeria tenella and the chicken immune response, chicken macrophage cell cultures of cell line HD-11 were infected with Eimeria tenella strain Hougton sporozoites. Samples were taken at 0, 2, 4, 12, 24, 48 and 72 hours post-infection and, purified and mRNA sequenced. A dual-RNA seq analysis was carried out, comparing the expression of infected chicken macrophages with uninfected ones at the same time points post-infection and comparing E. tenella samples during the infection with a sample of pure sporozoites. The results show a variety of response signals in the chicken, both previously known and unknown, as well as a clear role for different sets of SAG and MIC proteins for sporozoites and merozoites of E. tenella

2021-04-28 | GSE154393 | GEO

Eimeria tenella genome

Project description:Eimeria tenella Houghton annotated genome assembly

| PRJEB4863 | ENA

Eimeria tenella

Project description:Transcriptional profiling between virulent and precocious strains of Eimeria tenella

| PRJDB3854 | ENA