Project description:We conducted label-free, quantitative proteomics on Lasius niger queen spermathecal fluid and whole-body extracts, as well as Bombus terrestris queen spermathecal fluid and hemolymph, to investigate patterns of protein expression linked to sperm viability and reproductive stage (unmated, recently mated, and 1 week after emergence of first workers). All samples were analyzed on a timsTOF Pro 2 instrument except the L. niger spermathecal fluid, which was analyzed on a timsTOF SCP instrument. The "Compiled_data.xlsx" file contains both the sample metadata and search result tables for all experiments.
Project description:Genomic and proteomic characterization of the Aspergillus niger isolate, JSC-093350089, collected from U.S. segment surfaces of the International Space Station (ISS) is reported, along with a comparison to the experimentally established strain ATCC 1015. Whole-genome sequencing of JSC-093350089 revealed enhanced genetic variance when compared to publicly available sequences of A. niger strains. Analysis of the isolate’s proteome revealed significant differences in the molecular phenotype of JSC-093350089, including increased abundance of proteins involved in the A. niger starvation response, oxidative stress resistance, cell wall integrity and modulation, and nutrient acquisition. Together, these data reveal the existence of a distinct strain of A. niger onboard the ISS and provide insight into the molecular phenotype that is selected for by melanized fungal species inhabiting spacecraft environments.
Project description:Aspergillus niger is an opportunistic pathogen commonly found in a variety of indoor and out-door environments. Environmental isolates of A. niger taken from a pig farm were resistant to itraconazole and in-depth investigations were conducted to better understand cellular re-sponses during growth when exposed to an antifungal. Using a combination of cultivation techniques, antibiotic stress-testing, and label-free pro-teomics, this study has investigated the physiological and metabolic responses of A. niger to differing levels of antifungal stress.
