Bemisia tabaci
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA179139 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR611714.fastq.gz | Fastqsanger.gz | |||
| SRR611715.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:We investigated the transcriptional response to thiamethoxam in the Bemisia tabaci using Illumina sequencing technology. A total of 1,338 genes were differently expressed in the thiamethoxam-resistant whiteflies.
2013-07-15 | GSE42102 | GEO
Project description:To investigated the stage-specific gene expression response to thiamethoxam in the Bemisia tabaci, we have designed the Agilent eArray platform to identify stage-regulated gene expression towards thiamethoxam exposure.
2013-11-01 | GSE42337 | GEO
Project description:The whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), and the viruses it transmits, are a major constraint to vegetable crops, worldwide. Although the whitefly is usually controlled using chemical pesticides, biological control agents constitute an important component in integrated pest management programs. One of these agents is the wasp Eretmocerus mundus (Mercet) (Hymenoptera: Aphelinidae). E. mundus lays its egg on the leaf underneath the pupa of B. tabaci. First instars of the wasp hatch and penetrate the whitefly larvae. Initiation of parasitization induces the host to form a cellular capsule around the parasitoid. Around this capsule, epidermal cells multiply and thick layers of cuticle are deposited. The physiological and molecular processes underlying B. tabaci-E. mundus interactions have not been investigated so far. We have used a cDNA microarray containing 6,000 expressed sequence tags (ESTs) from the whitefly genome to study the parasitoid-whitefly interaction. We compared RNA samples collected across two time points of the parasitization process: when the parasitoid first instar started the penetration process and once it had fully penetrated the host. The results clearly indicated that genes known to be part of the defense pathways described in other insects are also involved in the response of B. tabaci to parasitization by E. mundus. Some of the responses observed included the repression of a serine protease inhibitor (serpin) and the induction of a melanization cascade. A second set of genes that strongly responded to parasitization included bacterial genes encoded by whitefly symbionts. Quantitative real-time PCR and FISH analyses showed that proliferation of Rickettsia, a facultative secondary symbiont, was strongly induced following the initiation of the parasitization process, a result that supported previous reports suggesting that endosymbionts may be involved in the insect host resistance to various environmental stresses. This is the first study examining the transcriptional response of a hemipteran insect to the attack of a biological control agent (Hymenopterous parasitoid), using a new genomic approach developed for this insect pest. The defense response in B. tabaci seems to resemble that of model organisms such as Drosophila melanogaster. Moreover, endosymbionts of B. tabaci seem to play a role in the response to parasitization, and this is supported by previously published results from aphids. Keywords: time course
2008-05-13 | GSE11410 | GEO
Project description:To investigated the stage-specific gene expression response to thiamethoxam in the Bemisia tabaci, we have designed the Agilent eArray platform to identify stage-regulated gene expression towards thiamethoxam exposure. All the B biotype Bemisia tabaci were maintained on cabbage. Thiamethoxam susceptible (TH-S) was cultured without exposure to any chemical insecticides, Thiamethoxam resistance (TH-R) strain exhibited >70-fold resistance to thiamethoxam in comparision to the TH-S strain. Eggs were incubated in 24hours collected as one sample. The fourth nymphs were collected as another sample. The one-day-old unmated adult females were collected as the third samples. Both of the samples were collected from the TH-R, TH-S strains, respectively
2013-11-01 | E-GEOD-42337 | biostudies-arrayexpress
Project description:We investigated the transcriptional response to thiamethoxam in the Bemisia tabaci using Illumina sequencing technology. A total of 1,338 genes were differently expressed in the thiamethoxam-resistant whiteflies. All the B biotype whiteflies were maintained on cabbage. The susceptible strain (TH-S) was cultured without exposure to any chemical insecticides. Before the sample collected, almost 10,000 thiamethoxam-resistant adult whiteflies were treated with 2,000 mg/L thiamethoxam (~LC80) to eliminate the heterozygous individuals. Then the survivors were collected after 48 hours and designated as the TH-2000 sample. Then approximately 1,500 adult TH-S and TH-2000 were collected, respectively, The RNA was extracted and sequenced using Illumina HiSeq 2000.
2013-07-15 | E-GEOD-42102 | biostudies-arrayexpress
Project description:The whitefly Bemisia tabaci is a notorious pest of worldwide agriculture. It is believed to secrete saliva to counter plant defenses, but the underlying mechanism remains to be elucidated. Here, we characterize the gene/protein repertoires of B. tabaci salivary glands and secreted saliva by transcriptomic and LC–MS/MS analysis. A total of 698 salivary gland-higher expressed unigenes, as well as 172 saliva proteins are identified. Comparative analysis of the saliva composition in different arthropod species illustrates that proteins associated with binding, hydrolysis and oxidation-reduction are widely distributed in herbivorous saliva. There are 74 saliva proteins exclusively identified in B. tabaci, with 34 of them being B. tabaci-specific. In addition, eleven B. tabaci-specific saliva proteins plastically regulated in response to different diets, which might be associated with wide host range of this pest. Our results gain insight into whitefly–plant interactions, and provide a good resource for functional characterization of effectors
2020-01-15 | PXD016581 | Pride
Project description:The whitefly Bemisia tabaci (Gennadius) causes tremendous losses to agriculture by direct feeding on plants and by vectoring several families of plant viruses. The B. tabaci species complex comprises over 10 genetic groups (biotypes) that are well defined by DNA markers and biological characteristics. B and Q are amongst the most dominant and damaging biotypes, differing considerably in fecundity, host range, insecticide resistance, virus vectoriality, and the symbiotic bacteria they harbor. We used a spotted B. tabaci cDNA microarray to compare the expression patterns of 6,000 ESTs of B and Q biotypes under standard 25°C regime and heat stress at 40°C. Overall, the number of genes affected by increasing temperature in the two biotypes was similar. Gene expression under 25ºC normal rearing temperature showed clear differences between the two biotypes: B exhibited higher expression of mitochondrial genes, and lower cytoskeleton, heat-shock and stress-related genes, compared to Q. Exposing B-biotype whiteflies to heat stress was accompanied by rapid alteration of gene expression. For the first time, the results here present differences in gene expression between very closely related and sympatric B. tabaci biotypes, and suggest that these clear-cut differences are due to better adaptation of one biotype over another and might eventually lead to changes in the local and global distribution of both biotypes.
2009-08-13 | GSE17567 | GEO
Project description:Transcriptome sequencing of whitefly (Bemisia tabaci) MEAM1
| PRJNA312467 | ENA
Project description:The whitefly, Bemisia tabaci, a notorious agricultural pest, has complex relationships with diverse microbes. It recognizes and degrades pathogens, as other insects do, and also relies on endosymbionts for its survival. Both types of interaction have received great attention, because of their potential importance in developing novel whitefly control technologies. The recent developments in RNA-seq technology allows us to perform a comprehensive investigation of a whitefly’s defense responses after it has ingested the pathogen, Pseudomonas aeruginosa. Compared to uninfected whiteflies, 6 and 24 hour post-infected (hpi) whiteflies showed 1,348 and 1,888 differentially expressed genes, respectively. Functional analysis highlighted the involvement of mitogen associated protein kinase (MAPK) pathway in host-defense regulation. Three knottin-like antimicrobial peptide genes and several components of the humoral and cellular immune response were also activated, indicating that key immune elements recognized in other insect species are also important for the host response of B. tabaci. Our data also suggest that intestinal stem cell mediated epithelium renewal might be an important component of the whitefly’s defense against oral bacterial infection. In addition, we also show stress responses to be an essential component of the defense system. We identify for the first time the key immune-response elements utilized by B. tabaci against bacterial infection. This provides a framework for future research into the complex interactions between whiteflies and microbes.
2014-12-01 | GSE52837 | GEO
Project description:The whitefly Bemisia tabaci (Gennadius) causes tremendous losses to agriculture by direct feeding on plants and by vectoring several families of plant viruses. The B. tabaci species complex comprises over 10 genetic groups (biotypes) that are well defined by DNA markers and biological characteristics. B and Q are amongst the most dominant and damaging biotypes, differing considerably in fecundity, host range, insecticide resistance, virus vectoriality, and the symbiotic bacteria they harbor. We used a spotted B. tabaci cDNA microarray to compare the expression patterns of 6,000 ESTs of B and Q biotypes under standard 25°C regime and heat stress at 40°C. Overall, the number of genes affected by increasing temperature in the two biotypes was similar. Gene expression under 25ºC normal rearing temperature showed clear differences between the two biotypes: B exhibited higher expression of mitochondrial genes, and lower cytoskeleton, heat-shock and stress-related genes, compared to Q. Exposing B-biotype whiteflies to heat stress was accompanied by rapid alteration of gene expression. For the first time, the results here present differences in gene expression between very closely related and sympatric B. tabaci biotypes, and suggest that these clear-cut differences are due to better adaptation of one biotype over another and might eventually lead to changes in the local and global distribution of both biotypes. 3 replicates comparing Q biotype under 40 degrees celsius (C) with three replicates under 25 C. The same number of replicates comparing B biotype under 40 C and 25 C, and three replicates comparing B and Q biotypes under 25 C.
2009-08-13 | E-GEOD-17567 | biostudies-arrayexpress