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Silanimonas lenta

ABSTRACT: Silanimonas lenta overview

PROVIDER: PRJNA185460 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
		Other
	AUBD01.dat.gz	Other
	AUBD01.fasta.gz	Fasta.gz
	AUBD01.master.dat	Other
	SRR896296_1.fastq.gz	Fastqsanger.gz

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1 - 5 of 6

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Gene expression profile of Eggerthella lenta DSM 2243 during growth in defined media EDM1

Project description:We grew E. lenta in chemically defined media, added one of multiple potential nutrients, and collected RNA during late exponential phase to determine both the overall profile of highly expressed metabolic enzymes and the gene expression response to nutrient conditions..

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Eggerthella lenta DSM 2243 alleviates bile acid stress response in Clostridium ramosum and Anaerostipes cacae by transformation of bile acids

Project description:Bile acids are not only crucial for the uptake of lipids, but also have widespread systematic ef-fects and shape the gut-microbiome composition. Bile acids can directly shape the gut-microbiome and can be modified by bacteria such as Eggerthella lenta which in turn plays a crucial role in host metabolism and immune response. We cultivated eight strains that represent a simplified human intestinal microbiome and inves-tigated the molecular response to bile acids, co-culturing with Eggerthella lenta and the combina-tion. We observed growth inhibition of particularly gram-positive strains during bile acid stress, which could be alleviated through co-culturing with Eggerthella lenta. The inhibition of growth was related to a decrease in membrane integrity and genotoxic effects of bile acids, which we investigated using zeta potential measurements in combination with proteomic and metabolomic analyses. Co-culturing with Eggerthella lenta alleviated stress through formation of oxidized and epimer-ized bile acids and the molecular response to co-culturing was seen to be strain specific. We also note that we could detect the recently described Microbial Bile Salt Conjugates in our cultures. This study highlights the significance of a potent bile acid modifier and how in-depth molecular analyses are required to decipher cross-communication between gut and host.

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Eggerthella lenta strain:C592

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Effects of the human intestinal bacterium Eggerthella lenta on the liver proteome of gnotobiotic mice.

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