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Pectobacterium atrosepticum [Delta]HAI2-SCRI1043

ABSTRACT: Pectobacterium atrosepticum [Delta]HAI2-SCRI1043 Genome sequencing and assembly

PROVIDER: PRJNA185466 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	AODV01.dat.gz	Other
	AODV01.fasta.gz	Fasta.gz
	AODV01.master.dat	Other
	AODV01.dat.gz	Other

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Pectobacterium atrosepticum [Delta]HAI2-SCRI1043

Project description:Pectobacterium atrosepticum [Delta]HAI2-SCRI1043 Genome sequencing

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Transcriptome microarray of wildtype, relA, expI and rpoS Pectobacterium atrosepticum strains in PMB media

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Transcriptome microarray of wildtype, relA, expI and rpoS Pectobacterium atrosepticum strains in PMB media

Project description:Whole genome analysis of gene expression by Pectobacterium atrosepticum strain SCRI1043 wildtype and its relA, expI and rpoS deletion mutants when grown to exponential and stationary phase in PMB media. The data is further described in Bowden et al (2013) Virulence in Pectobacterium atrosepticum is regulated by a coincidence circuit involving quorum sensing and the stress alarmone, (p)ppGpp. Molecular Microbiology. DOI: 10.1111/mmi.12369 A 24 chip study using total RNA recovered from three separate wild-type cultures of Pectobacterium atrosepticum SCRI1043 and three separate cultures from three single mutant strains of SCRI1043 possessing deletions within relA (ECA3569), expI (ECA0105) or rpoS (ECA3530) when grown in Pel Minimal Broth (PMB) media to log-phase (6h) or early stationary phase (14h) growth. Each chip measures the expression level of 4,472 genes from Pectobacterium atrosepticum SCRI1043 with eight 60-mer probe pairs (PM/MM) per gene, with three-fold technical redundancy.

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Project description:High-throughput sequencing of expanded CRISPR arrays in Pectobacterium atrosepticum

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Project description:Genome-wide in planta determination of the quorum sensing regulon in Pectobacterium atrosepticum, through gene expression analysis of ExpI mutants using Agilent custom microarrays.

2008-07-01 | E-TABM-384 | biostudies-arrayexpress

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Project description:Pectobacterium atrosepticum Genome sequencing

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Project description:Discovery and profiling of small RNAs responsive to stress conditions in the plant pathogen Pectobacterium atrosepticum

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Total mRNA sequence dataset from Pectobacterium atrosepticum colonising potato or radish roots

Project description:Pectobacterium atrosepticum (Pba) is a gram-negative bacterium which causes blackleg and tuber soft rot on potato. To investigate the molecular processes and responses involved in Pba-host (potato) and Pba-non-host (radish) interactions, under laboratory conditions, we used total RNA-sequencing to measure the gene expression patterns from all three species. Samples from infected and non-infected plant roots were collected after fourteen days of inoculation with Pba SCRI_1039 and subjected to total RNA-sequencing on an Illumina sequencing platform.

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