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Biofilter metagenome

ABSTRACT: Linuron contamination of an on-farm biopurification system

PROVIDER: PRJNA193662 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR807823.fastq.gz	Fastqsanger.gz
	SRR807848.fastq.gz	Fastqsanger.gz
	SRR807873.fastq.gz	Fastqsanger.gz
	SRR807874.fastq.gz	Fastqsanger.gz
	SRR807875.fastq.gz	Fastqsanger.gz

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Project description:These studies aimed to investigate the hepatic transcriptional response of brown trout to the natural estrogen, E2, and the herbicide linuron. We exposed mature male brown trout to three concentrations of each chemical for 4 days and sequenced the hepatic transcriptome of 3 individuals per treatment group in order to determine the global mechanisms of toxicity of these environmental contaminants. We assembled the brown trout transcriptome using a de novo approach. Subsequent differential expression analysis identified a total of 2113 differentially-regulated transcripts in the group exposed to the highest E2 treatment concentration, and 822 differentially-regulated transcripts across all linuron treatments. For E2, differentially-expressed transcripts included those encoding known oestrogen-responsive genes, while regulated processes included those associated with vitellogenesis including lipid metabolism, cellular proliferation and ribosome biogenesis. For linuron, there was a striking down-regulation of transcripts encoding the majority of the enzymes involved in the cholesterol biosynthesis pathway, and also a considerable induction of transcripts involved in cellular stress response including Cyp1a.

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