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Pinctada imbricata

ABSTRACT: Digital gene expression tag profile of five development stages of pearl sac from pearl oyster Pinctada martensii reveals potential candidates contributing to nacre formation

PROVIDER: PRJNA198491 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR833694.fastq.gz	Fastqsanger.gz
	SRR833695.fastq.gz	Fastqsanger.gz
	SRR833696.fastq.gz	Fastqsanger.gz
	SRR833697.fastq.gz	Fastqsanger.gz
	SRR833698.fastq.gz	Fastqsanger.gz

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cDNA microarray analysis revealing candidate biomineralizational genes of the pearl oyster, Pinctada fucata martensii

Project description:In order to screen and identify biomineralization gene, microarray technique was used to reveal tissues specific expression genes in the brunet mantle edge (ME), mantle centre (MC), and both ME and MC (ME-MC) from assembled transcriptome contigs of Pinctada fucata martensii, ideal pearl oyster for the study of biomineralization. Tissues of ME, MC, hepatopancreas, foot, gill, adductor muscle, heart and intestine were sampled from two females and one male pearl oyster. Gonad was sampled from above three individuals and another two male and one female. Equal amount RNA of each individual hepatopancreas, foot, gill, adductor muscle, heart and intestine were mixed as a composite viscera sample (CV), and equal amount gonad RNA from one male and one female were combined together as a gonad sample (GS). Hybridizations were performed with twelve samples of ME, MC, CV and GS.

2016-07-01 | GSE70439 | GEO

cDNA microarray analysis revealing candidate biomineralizational genes of the pearl oyster, Pinctada fucata martensii

2016-07-01 | E-GEOD-70439 | biostudies-arrayexpress

miRNA expression profile of mantle tissues for pearl oyster, Pinctada fucata

Project description:To elucidate the modulatory participation of miRNAs in mollusk biomineralization, we have employed high-throughput sequencing to identify miRNAs of pearl oyster, Pinctada fucata. Our study focused on the miRNA expression profile of the mantle, an organ responsible for shell formation of the oyster. The pearl oysters were cultured in the tank with the maintaining conditions of temperature 19 ℃, PH 8.1 and salinity 33‰ in recirculating seawater.

2021-05-01 | GSE145993 | GEO

Pinctada imbricata

Project description:Pinctada martensii overview

| PRJNA320329 | ENA

Pinctada imbricata

Project description:Mantle of Pinctada fucata martensii

| PRJNA338372 | ENA

Global gene expression profile for pearl oyster

Project description:This SuperSeries is composed of the following subset Series: GSE13980: Analysis of the global gene expression profile for pearl oyster, Pinctada maxima, exposed to organotin (tributyltin) GSE14303: Differential expression analysis of genes from the mantle tissue of pearl oyster: Pinctada maxima GSE14305: The microstructural, mineralogical and transcriptional developments of shell biomineralization of Pinctada maxima Refer to individual Series

2010-02-04 | E-GEOD-14306 | biostudies-arrayexpress

Pinctada fucata martensii mantle edge tissue LC-MS/MS

Project description:We have got a yellow shell variety of Pinctada fucata martensii after years of artificial breeding. To identify differentially expressed genes between yellow shell and normal black shell pearl oysters, we performed label-free proteomic analyses by LC-MS using mantle edge tissues.

2019-11-13 | PXD012211 | Pride

Pinctada imbricata strain:pearl sac

Project description:Pinctada imbricata strain:pearl sac Transcriptome or Gene expression

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Pinctada fucata martensii shell matrix protein LC-MSMS

Project description:Nacre, the iridescent material found in pearls and shells of molluscs, is formed through an extraordinary process of matrix-assisted biomineralization. Despite recent advances, many parts of the biomineralization process and its evolutionary origin remain a mystery. The pearl oyster Pinctada fucata martensii is a well-known master of biomineralization, but the molecular mechanisms underlie its production of remarkable shells and pearls is not fully understood. We sequenced the highly polymorphic genome of the pearl oyster and conducted multi-omic and biochemical studies to probe nacre formation. We identified a large set of novel proteins participating in matrix-framework formation, many in expanded families, including components similar to that found in vertebrate bones such as collagen-related VWA-containing proteins (VWAP), chondroitin sulfotransferases and regulatory elements.Considering that there are only collagen-based matrices in vertebrate bones and chitin-based matrices in most invertebrate skeletons, the presence of both chitin and elements of collagen-based matrices in nacre matrices suggests that elements of chitin- and collagen-based matrices are deeply rooted and might be part of an ancient biomineralizing matrix. Our results expand the current shell matrix-framework model and provide new insights into the evolution of diverse biomineralization systems.

2017-07-07 | PXD006786 | Pride

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Project description:Pinctada fucata martensii mantle edge tissues Raw sequence reads

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