Project description:We used RNA-Seq to assess how the presence/absence of the RD2 pathogenicity island influences global gene expression in the parental serotype M1 GAS isolate MGAS2221 and the parental serotype M49 isolate NZ131
Project description:The human-derived serotype Ⅴ ST1 GBS strains NNA038 and NNA048 was isolated from a amniotic fluid of full-term pregnant woman who suffered from premature rupture of membrane in China.Serotype Ia ST7 GBS strain YM001 is an attenuated strain ,its parent strain HN016 was isolated from an outbreak epidemical disease in tilapia from China.HN016_KO_D2 is a knockout strain from Serotype Ia ST7 GBS strain HN016.
Project description:The proteome and phosphoproteome of Streptococcus pyogenes M49 was investigated at different growth phases of cultures grown in either rich medium (THY), chemically defined medium without carbon source, or chemically defined medium containing 1% fructose. Of the 815 phosphosites identified, 463 were included in a label-free quantitative analysis of dynamic protein phosphorylation. A small group of phosphorylation events, almost exclusively at threonine residues, was strongest during growth and decreased during stationary phase. These included phosphorylation sites of the PASTA kinase SP-STK and its putative substrates suggesting that the PASTA kinase-dependent processes regulating the cell cycle in related bacteria are also conserved in S. pyogenes. Most phosphorylation events occurred preferentially at serine residues in the stationary growth phase and under starvation conditions. The elucidation of the physiological significance and the responsible kinases of these phosphorylations require further investigations. In addition to phosphorylation events at S/T/Y residues, phosphoglycerylation of lysine (PGK) occurred frequently among the enriched phosphopeptides.