Project description:The complete mitochondrial genome of Liobagrus styani was sequenced by the long and accurate polymerase chain reaction and primer walking sequence method, and each partition was characterized. This genome, with 16,515?bp in length, includes 13 protein-coding genes, 22 tRNA genes, 2rRNA genes, and 2 non-coding regions. Genes encoding on the genome are similar among all vertebrates. These genes except ND6 and 8 tRNA genes were encoded on the H-strand. Phylogenetic relationship of this species with other congeners was inferred using Bayesian Inference methods based on the genome. Result is contrast to traditional biogeographic explanation for faunal similarity and highlight the need for a further investigation on the formation of allopatric distribution pattern of the genus Liobagrus in East Asia.
Project description:The south torrent catfish Liobagrus mediadiposalis (Teleostei, Siluriformes, Amblycipitidae) is an endemic species of Korea. The complete mitochondrial genome sequence consisted of 16,534 base pairs (bp) encoding 13 protein-coding genes (PCGs), two ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs) and two non-coding regions. The overall base composition of L. mediadiposalis was G + C: 44.8%, A + T: 55.2%, apparently with a slight AT bias. Phylogenetic analysis showed that L. mediadiposalis was closely related to Liobagrus reinii.
Project description:The Korean torrent catfish (Liobagrus andersoni), an endemic species in Korea, is a member of the family Amblycipitidae. Herein, we report the first sequencing and assembly of the complete mitochondrial genome of L. andersoni. The complete mitochondrial genome is 16,514 bp long, consisting of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and a control region. It has the typical vertebrate mitochondrial gene arrangement. Phylogenetic analysis using mitochondrial genomes of 21 species showed that L. andersoni was clustered with L. marginatoides and L. nigricauda. This mitochondrial genome provides potentially important resources for addressing taxonomic issues and studying molecular evolution.
Project description:The Korean catfish, Liobagrus somjinensis, was recorded in 2010 as a new species of genus Liobagrus. The complete mitochondrial DNA sequence of L. somjinensis was sequenced by next-generation sequencing (NGS) analysis. The assembled mitogenome was 16,526 bp in length and encoded 13 protein-coding genes (PCGs), 22 tRNAs, and 2 rRNAs. The gene arrangement, content, and total size were clearly identical with the congeneric species, L. mediadiposalis. Phylogenetic analysis based on nucleotide dataset, consisting PCGs and rRNA genes revealed the taxonomical relationship in species level among the genus Liobagrus.
Project description:Liobagrus hyeongsanensis, Korean indigenous catfish, was reported as a new species in 2015. The complete mitochondrial DNA sequence of L. hyeongsanensis was sequenced by next-generation sequencing (NGS) analysis. The mitochondrial genome was assembled with 16,529 bp in length and encoded 13 protein-coding genes (PCGs), 22 tRNAs, two rRNAs, and one control region (D-loop). Also, the gene structures such as gene order and content were totally identical with the congeneric species. Molecular phylogenetic analysis determined the taxonomical position of L. hyeongsanensis in species level among the genus Liobagrus.
