ABSTRACT: 16SrRNA gene - 454 pyrosequencing. Microbiome (eubacteria) study of activated sludge from wastewater treatment plants and from microbial enrichments promoting anammox process
Project description:16SrRNA gene - 454 pyrosequencing. Microbiome (eubacteria) study of activated sludge from wastewater treatment plants and from microbial enrichments promoting anammox process
Project description:High-throughput sequencing of Arabidopsis thaliana endogenous small RNAs by 454 pyrosequencing. Keywords: high-throughput sequencing
Project description:Anaerobic ammonium oxidizing (anammox) bacteria mediate a key step in the biogeochemical nitrogen cycle and have been applied worldwide for the energy-efficient removal of nitrogen from wastewater. However, outside their core energy metabolism, little is known about the metabolic networks driving anammox bacterial anabolism and mixotrophy beyond genome predictions. Here, we experimentally resolved the central carbon metabolism using metabolomics (LC-MS and GC-MS), metabolic flux analysis and proteomics (shot-gun proteomics).
Project description:Characterization of gene expression levels with RNA-seq was performed on self-renewing (SR) and senescent (SEN) human adult adipose derived mesenchymal stem cells (hADSCs) using the Roche 454 pyrosequencing platform.
Project description:Assessing the structure of microbial communities in textile wastewater treatment plants in comparison with municipal wastewater treatment plants
