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Pseudomonas donghuensis strain:P482

ABSTRACT: Pseudomonas donghuensis P482 Genome sequencing

PROVIDER: PRJNA239153 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	JHTS01.dat.gz	Other
	JHTS01.fasta.gz	Fasta.gz
	JHTS01.master.dat	Other
	JHTS01.dat.gz	Other

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Effect of supplementation of M9 0.4% glucose with root exudates of either tomato or maize on gene expression in WT Pseudomonas donghuensis P482

Project description:To investigate and compare the influence of root exudates of tomato and maize on Pseudomonas donghuensis P482, we have grown the strain up to a stationary phase in M9 0.4% glucose medium supplemented with maize exudates (Maize), tomato exudates (Tomato) or without supplementation (Control). We then performed differential gene expression analysis, identifying changes in transcriptome profiles between each treatment (Tomato, Maize) and the Control as reference conditions, and between the two treatments.

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GNPS - Pseudomonas donghuensis - fungistatic metabolite - Kinetic profile

Project description:Non-targeted metabolomics comparative study of Pseudomonas donghuensis SVBP6 WT and mutant bacteria strains.

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Transcriptomic analysis of bacterial strains in the cbr/crc regulatory system

Project description:We screened a number of virulence genes in Pseudomonas donghuensis HYS, and genes in cbr/crc regulatory system were included. To investigate the relationship between this regulatory system and other virulence genes screened in the study, we performed transcriptome sequencing of strains related to cbr/crc system. Through analyzing the expression quantity, we found that the screened virulence genes were under the regulation of the cbr/crc system.

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DNA Methyltransferase M.PaeTBCFI in a chronically adapted Pseudomonas aeruginosa strain

Project description:Pseudomonas aeruginosa is an opportunistic pathogen which causes acute and chronic infections that are difficult to treat. Comparative genomic analysis has showed a great genome diversity among P. aeruginosa clinical strains and revealed important regulatory traits during chronic adaptation. While current investigation of epigenetics of P. aeruginosa is still lacking, understanding the epigenetic regulation may provide biomarkers for diagnosis and reveal important regulatory mechanisms. The present study focused on characterization of DNA methyltransferases (MTases) in a chronically adapted P. aeruginosa clinical strain TBCF10839. Single-molecule real-time sequencing (SMRT-seq) was used to characterize the methylome of TBCF. RCCANNNNNNNTGAR and TRGANNNNNNTGC were identified as target motifs of DNA MTases, M.PaeTBCFI and M.PaeTBCFII, respectively.

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Determination of carbon metabolism response regulators regulons in several Pseudomonas species by DAP-seq

Project description:We identified binding sites genome-wide for carbon metabolism transcriptional response regulators in Pseudomonas stutzeri RCH2, Pseudomonas putida KT2240, Pseudomonas fluorescens FW300-N2E2 and FW300-N2C3 by DNA-affinity-purified (DAP) sequencing.

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Genomic insights into the broad antifungal activity, plant-probiotic properties, and their regulation, in Pseudomonas donghuensis strain SVBP6.

Project description:Plant-growth promotion has been linked to the Pseudomonas genus since the beginning of this research field. In this work, we mined the genome of an Argentinean isolate of the recently described species P. donghuensis. Strain SVBP6, isolated from bulk soil of an agricultural plot, showed a broad antifungal activity and several other plant-probiotic activities. As this species has been recently described, and it seems like some plant-growth promoting (PGP) traits do not belong to the classical pseudomonads toolbox, we decide to explore the SVBP6 genome via an bioinformatic approach. Genome inspection confirmed our previous in vitro results about genes involved in several probiotic activities. Other genetic traits possibly involved in survival of SVBP6 in highly competitive environments, such as rhizospheres, were found. Tn5 mutagenesis revealed that the antifungal activity against the soil pathogen Macrophomina phaseolina was dependent on a functional gacS gene, from the regulatory cascade Gac-Rsm, but it was not due to volatile compounds. Altogether, our genomic analyses and in vitro tests allowed the phylogenetic assignment and provided the first insights into probiotic properties of the first P. donghuensis isolate from the Americas.

| S-EPMC5851621 | biostudies-literature

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