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Variation in primary CD4+ T cell response to activation across time and people

ABSTRACT: Variation in primary CD4+ T cell response to activation across time and people

PROVIDER: PRJNA257800 | ENA |

REPOSITORIES: ENA

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Variation in primary CD4+ T cell response to activation across time and people

Project description:This SuperSeries is composed of the SubSeries listed below. Variation in individuals' adaptive immune response is believed to influence susceptibility to complex diseases in humans. The genetic basis of such variation is poorly understood. We analyzed the response of human CD4+ T cells from a cohort of 348 healthy humans, representing three major ancestries, to in vitro stimulation, alone or in Th17 conditions, or with IFNb stimulation.

2014-08-28 | GSE60236 | GEO

Variation in primary CD4+ T cell response to activation across time and people

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-08-28 | E-GEOD-60236 | biostudies-arrayexpress

Transcription profiling of human primary CD4+ T cell activation, time series

Project description:Little is known about the global transcriptional program underlying CD4+ T-cell activation. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of human CD4+ T-cell activation. The goal of this study was to identify genes involved in the various facets of human CD4+ T-cell activation. Experiment Overall Design: CD4+ T cells isolated from peripheral blood were cultured with CD3, CD28, with or without IL-2 to induce T-cell activation. At each timepoint, cells were harvested and frozen for RNA isolation. Three biological replicate experiments were analyzed and approximately one-half of the samples from each experiment were technically replicated. Hybridizations were performed in a reference design with all samples labeled with Cy3 and a reference thymus total RNA labeled with Cy5.

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Temporal expression profile of primary CD4+ T cell activation

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MicroArray time course transcriptional profiling of Human CD4+ T Cell activation across 20 donors.

Project description:CD4+ T cells are at the centre of the adaptive immune system and can differentiate into distinct states that maintain self-tolerance and guide specific humoral and cellular responses to infection. Perturbation of CD4+ T cell activation is associated with many human diseases, including autoimmunity, therefore characterising the transcriptional programs underlying this process is key to the development of future therapeutic interventions. To assess the gene expression on CD4+ T cells, we recruited 20 healthy volunteers from the Cambridge BioResource. Total CD4+ T cells were isolated from whole blood within 2 hours of venepuncture. To assess the transcriptional variation in response to TCR stimulation, 1 million CD4+ T cells were cultured in U-bottom 96-well plates in the presence or absence of human T activator CD3/CD28 beads. Cells were harvested at 2 , 4, 6 or 21 hours post-stimulation, or after 0, 6 or 21 hours in the absence of stimulation. Three samples from the 6 hour unstimulated timepoint were omitted from the study due to insufficient cell numbers, and a further four samples were dropped after quality control, resulting in a total of 133 samples that were included in the final analysis.

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Temporal expression profile of primary CD4+ T cell activation

Project description:Temporal expression profile of primary CD4+ T cell activation

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Expression data from different levels of anti-CD4 in HIV+ people

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Blood RNA sequencing in HIV-negative healthy volunteers and people living with HIV on effective, long-term anti-retroviral therapy

Project description:Immune activation in people living with HIV on anti-retroviral therapy is associated with increased risk of morbidity and mortality, but the underlying mechanisms are poorly understood. To identify whether perturbation of immunological pathways persist at systems level, we compared genome-wide whole blood transcriptomes from 26 people living with HIV on long-term anti-retroviral therapy with 12 HIV-negative healthy controls. All participants were Caucasian male adults recruited from London, UK. People living with HIV were on anti-retroviral therapy for a median of 8.5 years (interquartile range 3-16 years). They had undetectable plasma HIV viral load (<40 copies/ml) and median circulating CD4 counts of 703 cells/µl (interquartile range 491-841 cells/µl).

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