Project description:In this study, we identified the O-glycosylation sites in recombinant Clostridium botulinum flagellin (CbFla) and Geobacillus kaustophilus flagellins, GkFlaA1 and GkFlaA2, expressed from the E. coli strains, EV136 and EV240 (K1:K12 strains engineered by Prof. Eric Vimr’s group to accumulate CMP-sialic acid in the cytosol), EV36 (wildtype K1:K12 strain), and BL21(DE3) (laboratory strain used for protein expression using T7 RNA polymerase). The recombinant flagellins were expressed with or without co-expression of motility associated factor (Maf) (flagellin nonulosonic acid glycosyltransferase) in these strains, purified and subjected to MS/MS analysis at Taplin Biological Mass Spectrometry facility.

2020-09-07 | PXD018167 | Pride

Geobacillus kaustophilus GBlys

Project description:Genome sequence of G. kaustophilus GBlys

| PRJDB1127 | ENA

Draft Genome Sequence of Geobacillus kaustophilus GBlys, a Lysogenic Strain with Bacteriophage OH2.

Project description:Geobacillus kaustophilus strain GBlys was isolated along with the bacteriophage OH2, which infects G. kaustophilus NBRC 102445(T). Here we present a draft sequence of this strain's genome, which consists of 216 contigs for a total of 3,541,481 bp, 3,679 predicted coding sequences, and a G+C content of 52.1%.

| S-EPMC3744691 | biostudies-literature

Geobacillus thermoleovorans

Project description:Geobacillus thermoleovorans Genome sequencing

| PRJNA556596 | ENA

Geobacillus stearothermophilus

Project description:Geobacillus stearothermophilus Genome sequencing

| PRJNA464412 | ENA

Geobacillus

Project description:Geobacillus

| PRJNA27957 | ENA