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Identification of Tox chromatin binding properties and of its downstream targets by DamID

ABSTRACT: Identification of Tox chromatin binding properties and of its downstream targets by DamID

PROVIDER: PRJNA270582 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1720440.fastq.gz	Fastqsanger.gz
	SRR1720441.fastq.gz	Fastqsanger.gz
	SRR1720442.fastq.gz	Fastqsanger.gz
	SRR1720443.fastq.gz	Fastqsanger.gz
	SRR1720444.fastq.gz	Fastqsanger.gz

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Identification of Tox chromatin binding properties and of its downstream targets by DamID

Project description:Purpose: The goal of this study was to characterize the chromatin binding features and the transcriptional target genes of a new key player during corticogenesis, the transcription factor Tox Methods: DamID was performed using two cell lines, the Neuro-2a and the HEK-293T cells. Experiments were performed in duplicates for each cell line by infecting them with pLgwV5Eco-ToxDam lentiviruses and using the pLgxV5Dam viruses as control. DamID was performed as described in Vogel et al., 2006. Results: Our analysis identifyed ca. 13,000 Tox chromatin binding regions

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