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Dodonaea viscosa

ABSTRACT: Dodonaea viscosa Transcriptome or Gene expression

PROVIDER: PRJNA277994 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR1914332_1.fastq.gz	Fastqsanger.gz
	SRR1914332_2.fastq.gz	Fastqsanger.gz
	SRR1914333_1.fastq.gz	Fastqsanger.gz
	SRR1914333_2.fastq.gz	Fastqsanger.gz
	SRR1914334_1.fastq.gz	Fastqsanger.gz

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Co-cultivation and transcriptome sequencing of two co-existing fish pathogens Moritella viscosa and Aliivibrio wodanis.

Project description:Aliivibrio wodanis and Moritella viscosa have often been isolated together from fish with winter ulcer. Little is known about the interaction between the two bacterial species and how the presence of one bacterial species affects the behaviour of the other. The impact on bacterial growth in co-culture was investigated in vitro, and the presence of A. wodanis has a strong inhibitorial effect on M. viscosa. Further, we have sequenced the complete genomes of these two marine Gram-negative species, and have performed transcriptome analysis of the bacterial gene expression levels from in vivo samples. Using bacterial implants in the fish abdomen, we demonstrate that the presence of A. wodanis is altering the gene expression levels of M. viscosa compared to when the bacteria are implanted separately. The impeding effect on growth and the change in the global gene expression pattern of M. viscosa when the two pathogens co-exists is discussed in this paper.

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Project description:Bacterial pathogen Moritella viscosa, the causative agent of winter ulcer, causes heavy losses in Atlantic salmon aquaculture. The study compared responses in salmon reared under normal condition (G100) and fish exposed to hypoxia - 60% saturation of dissolved oxygen - at early life (G60). G60 showed lower survival after challenge. Analyses were performed in the most affected tissues: skin and spleen

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