Project description:Sixteen new prenylated flavonoids, sinoflavonoids P-Z (1-11) and sinoflavonoids NA-NE (12-16), were isolated from the fruit of Sinopodophyllum hexandrum, along with eight known analogues (17-24). Their structures were elucidated on the basis of extensive spectroscopic data (HR-ESI-MS, 1H-NMR, 13C-NMR, HSQC, HMBC). The cytotoxic activities of compounds 1-18, 20, and 22 were evaluated by MTT assay. Compound 6 showed the most potent cytotoxicity in MCF-7, and HepG2 cell lines, with IC50 values of 6.25 and 3.83 ?M, respectively.

| S-EPMC6749350 | biostudies-literature

Transcriptome sequencing of rhizome tissue of Sinopodophyllum hexandrum at two temperatures.

Project description:BACKGROUND: Sinopodophyllum hexandrum is an endangered medicinal herb, which is commonly present in elevations ranging between 2,400-4,500 m and is sensitive to temperature. Medicinal property of the species is attributed to the presence of podophyllotoxin in the rhizome tissue. The present work analyzed transcriptome of rhizome tissue of S. hexandrum exposed to 15°C and 25°C to understand the temperature mediated molecular responses including those associated with podophyllotoxin biosynthesis. RESULTS: Deep sequencing of transcriptome with an average coverage of 88.34X yielded 60,089 assembled transcript sequences representing 20,387 unique genes having homology to known genes. Fragments per kilobase of exon per million fragments mapped (FPKM) based expression analysis revealed genes related to growth and development were over-expressed at 15°C, whereas genes involved in stress response were over-expressed at 25°C. There was a decreasing trend of podophyllotoxin accumulation at 25°C; data was well supported by the expression of corresponding genes of the pathway. FPKM data was validated by quantitative real-time polymerase chain reaction data using a total of thirty four genes and a positive correlation between the two platforms of gene expression was obtained. Also, detailed analyses yielded cytochrome P450s, methyltransferases and glycosyltransferases which could be the potential candidate hitherto unidentified genes of podophyllotoxin biosynthesis pathway. CONCLUSIONS: The present work revealed temperature responsive transcriptome of S. hexandrum on Illumina platform. Data suggested expression of genes for growth and development and podophyllotoxin biosynthesis at 15°C, and prevalence of those associated with stress response at 25°C.

| S-EPMC4200142 | biostudies-literature

Podophyllum hexandrum Royle MS/MS

Project description:In order to study the molecular mechanisms of cold-adaption and PPT accumulation to understand why P. hexandrum adapts to chilling temperature, the differentially expressed proteins (DAPs) of P. hexandrum seedling exposed to chilling temperatures (4 and 10°C, 22°C as control) were conducted by using 2-DE in combination with MALDI-TOF-MS, a total of 35 DAP spots were detected and identified. Interestingly, 14 of the 35 DAPs played key roles in PPT biosynthesis, and 21 proteins participated in growth and development. These protein spots were classified into 7 functional categories, including PPT biosynthesis, primary metabolism, transcription and protein synthesis, cell growth/division, defense, transport, and energy. The present data would be useful in better understanding the molecular basis of chilling temperature response in P. hexandrum.

2017-05-15 | PXD003334 | Pride

Project description:Transcriptome sequencing of geographically separated Podophyllum hexandrum (syn: Sinopodophyllum hexandrum)

| PRJNA577552 | ENA