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Phelipanche aegyptiaca

ABSTRACT: Phelipanche aegyptiaca Raw sequence reads

PROVIDER: PRJNA296549 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR2477710_1.fastq.gz	Fastqsanger.gz
	SRR2477710_2.fastq.gz	Fastqsanger.gz
	SRR2477711_1.fastq.gz	Fastqsanger.gz
	SRR2477711_2.fastq.gz	Fastqsanger.gz
	SRR2477712_1.fastq.gz	Fastqsanger.gz

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Phelipanche aegyptiaca

Project description:Phelipanche aegyptiaca Raw sequence reads

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Phelipanche aegyptiaca cultivar:Phelipanche aegyptiaca

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Phelipanche aegyptiaca

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ABA signaling components in Phelipanche aegyptiaca.

Project description:Obligate root holoparasite Phelipanche aegyptiaca is an agricultural pest, which infests its hosts and feeds on the sap, subsequently damaging crop yield and quality. Its notoriously viable seed bank may serve as an ideal pest control target. The phytohormone abscisic acid (ABA) was shown to regulate P. aegyptiaca seed dormancy following strigolactones germination stimulus. Transcription analysis of signaling components revealed five ABA receptors and two co-receptors (PP2C). Transcription of lower ABA-affinity subfamily III receptors was absent in all tested stages of P. aegyptiaca development and parasitism stages. P. aegyptiaca ABA receptors interacted with the PP2Cs, and inhibited their activity in an ABA-dependent manner. Moreover, sequence analysis revealed multiple alleles in two P. aegyptiaca ABA receptors, with many non-synonymous mutations. Functional analysis of selected receptor alleles identified a variant with substantially decreased inhibitory effect of PP2Cs activity in-vitro. These results provide evidence that P. aegyptiaca is capable of biochemically perceiving ABA. In light of the possible involvement of ABA in parasitic activities, the discovery of active ABA receptors and PP2Cs could provide a new biochemical target for the agricultural management of P. aegyptiaca. Furthermore, the potential genetic loss of subfamily III receptors in this species, could position P. aegyptiaca as a valuable model in the ABA perception research field.

| S-EPMC6482195 | biostudies-literature

Genome-wide Identification of Lipoxygenases in Luffa aegyptiaca and Functional Characterization in Response to Abiotic Stress

Project description:RNA-seq of four Luffa aegyptiaca samples in response to heat stress

2024-10-13 | GSE275983 | GEO

Phelipanche ramosa

Project description:Phelipanche ramosa seed and soil metagenome

| PRJNA589539 | ENA

Luffa aegyptiaca

Project description:Luffa aegyptiaca Organism overview

| PRJNA324136 | ENA

Transformation and regeneration of the holoparasitic plant Phelipanche aegyptiaca.

Project description:BACKGROUND:Transformation and subsequent regeneration of holoparasitic plants has never been reported, in part due to challenges in developing transformation protocols, but also because regeneration of obligate parasites is difficult since their survival depends completely on successful haustorium penetration of a host and the formation of vascular connections. The recent completion of a massive transcriptome sequencing project (the Parasitic Plant Genome Project) will fuel the use of genomic tools for studies on parasitic plants. A reliable system for holoparasite transformation is needed to realize the full value of this resource for reverse genetics and functional genomics studies. RESULTS:Here we demonstrate that transformation of Phelipanche aegyptiaca is achieved by infection of 3 month-old in vitro grown P. aegyptiaca calli with Agrobacterium rhizogenes harboring the yellow fluorescent protein (YFP). Four months later, YFP-positive regenerated calli were inoculated onto tomato plants growing in a minirhizotron system. Eight days after inoculation, transgenic parasite tissue formed lateral haustoria that penetrated the host and could be visualized under UV illumination through intact host root tissue. YFP-positive shoot buds were observed one month after inoculation. CONCLUSIONS:This work constitutes a breakthrough in holoparasitic plant research methods. The method described here is a robust system for transformation and regeneration of a holoparasitic plant and will facilitate research on unique parasitic plant capabilities such as host plant recognition, haustorial formation, penetration and vascular connection.

| S-EPMC3285091 | biostudies-literature

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