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Mus musculus

ABSTRACT: ChIP-seq analysis of Blimp1 in day 2 differentiated mouse trophoblast stem cells.

PROVIDER: PRJNA300306 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2832850_1.fastq.gz	Fastqsanger.gz
	SRR2832850_2.fastq.gz	Fastqsanger.gz
	SRR2832851_1.fastq.gz	Fastqsanger.gz
	SRR2832851_2.fastq.gz	Fastqsanger.gz
	SRR2832852_1.fastq.gz	Fastqsanger.gz

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Project description:The establishment and function of the human placenta is dependent on specialized cells called trophoblasts. Unfortunately, little is known about the cellular and molecular processes controlling human trophoblast stem cell maintenance and differentiation into mature trophoblast sub-populations/cell states. To address this, we here report transcriptomic data from n=7 first trimester human placental tissues, n=3 regenerative human trophoblast stem cell (hTSC) derived trophoblast organoids, and n=3 EVT-differentiated hTSC derived organoid cultures at single-cell resolution. This study sought to identify the molecular programs and cell states important in trophoblast progenitor establishment, renewal, and differentiation. Placental tissues were collected following elective termination at the British Columbia Women’s Hospital CARE clinic. hTSC cultures were established as described and sequenced on either day 0 of culture (for regenerative organoid colonies), or day 7 of culture (for EVT-differentiated organoid colonies).

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